RT Journal Article
SR Electronic
T1 The Mechanism of Cisplatin-induced Apoptosis in HeLa Cells
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 866
OP 869
VO 2
IS 6
A1 Youqing Liu
A1 Hui Xing
A1 Xiaobing Han
A1 Xiaoyan Shi
A1 Fengqi Liang
A1 Gang Chen
A1 Ding Ma
YR 2005
UL http://www.cancerbiomed.org/content/2/6/866.abstract
AB OBJECTIVE To study the mechanism of apoptosis induced by cisplatin in vitro in HeLa cells cervical cancer cell line.METHODS AThe inhibitory effect of cisplatin on HeLa cell growth was analyzed by the MTT assay. Cell apoptosis was measured with flow cytometry and Hoechst 33258 staining following cisplatin treatment. The effect of cisplatin on transcription of HPV E6 was analyzed by RT-PCR and protein expression of E6, P53, p21, Bax and Bcl-2 was studied by Western blots.RESULTS Cisplatin inhibited cellular proliferation in a time and dose - dependant manner. The sub-G1 peak by flow cytometry showed a high-er apoptotic rate in the experimental group compared to the controls and Hoechst 33258 staining indicated that apoptosis was induced by cisplatin. Results of RT-PCR demonstrated that cisplatin decreased transcription of E6. Western Blots showed that cisplatin decreased protein expression of E6 and increased protein expression of P53, P21 and Bax but had no effect on protein expression of Bcl-2.CONCLUSION Cisplatin induces apoptosis and death of HeLa cells through the suppression of HPV E6 and restoration of p53 function.