RT Journal Article
SR Electronic
T1 The Effect Of Curcumin on Proliferation and Apoptosis in LNCap Prostate Cancer Cells
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 55
OP 60
VO 3
IS 1
A1 Lei Yang
A1 Lijun Chen
A1 Bin Meng
A1 Jiangrui Suo
A1 Hongmin Wang
A1 Hong Xie
A1 Qiuyue Jin
A1 Li Yao
A1 Ruimin Wang
A1 Lianying Zhang
YR 2006
UL http://www.cancerbiomed.org/content/3/1/55.abstract
AB Objective To observe the effect of curcumin on proliferation and apoptosis in the prostate cancer LNCaP cell line.Methods The AXSYMTM system luciferase method was used to examine the effect of various concentratious of curcumin on the content of prostate specific antigen (PSA) in prostate cancer LNCaP cells. A pGL3-PSA luciferase expression vector, containing 640 bp DNA of the PSA gene 5’ -promoter region was constructed and transfected into the LNCaP cells with lipofectin. By measuring luciferase activity, the effect of 10 μmol/L, 20 μmol/L, 30 and 40 μmol/L curcumin on the promoter was studied. Effects on cell, growth and apoptosis were analyzed by microscopy, the MTT colorimetric assay and flow cytometry. Western-blotting was used to measure expression of the androgen receptor (AR) in the LNCaP cells treated with different concentrations of curcumin.Results The results showed that the expression of PSA was inhibited as curcumin reduced the activity of luciferase. Curcumin also caused a sigificant concentration-dependent decrease in AR expession measured by Western -blotting. Cell growth was inhibited and apoptosis was induced.Conclusion By inhibiting AR expression, curcumin reduced the function of the PSA promoter and inhibited PSA protein expression. Curcumin decreased the cellular proliferation and induced apoptosis in LNCaP cells in a concention-dependent manner.