RT Journal Article
SR Electronic
T1 Phenylacetate Down-regulates Expression of RNA Editing Deaminase ADAR2 mRNA in U-251MG Glioma Cells
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 1
OP 4
VO 3
IS 1
A1 Yu Tian
A1 Yuzuo Pan
A1 Yufei Gao
A1 Guilin Li
A1 Jun Gao
A1 Xingli Zhao
A1 Guiying Li
A1 Renzhi Wang
YR 2006
UL http://www.cancerbiomed.org/content/3/1/1.abstract
AB OBJECTIVE To observe the effect of phenylacetate on the expression of RNA editing deaminase ADAR2 mRNA in glioma cells.METHODS Primary glial cells from human brain tissue and glioma U-251 MG cells were cultured. The expression of ADAR2 mRNA was detected by a semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The levels of ADAR2 mRNA expression before and after treatment with phenylacetate were tested by RT -PCR and image analysis. The level of ADAR2 gene expression was presented as the ratio expression rate (RER) of ADAR2 gene/β-actin based on computer image analysis.RESULTS The ADAR2 mRNA displayed mild expression in brain glial cells, and a high expression level in high-grade malignant U-251 MG glioma cells. Computer image analysis showed that the RERs of the ADAR2 gene in the U -251 MG cells before and after treatment with 4.0, and 5.0 mM phenylacetate for 8 h were 100.0, 73.5, 60.3, respectively. The expression of ADAR2 mRNA was decreased by phenylacetate in glioma U-251MG cells.CONCLUSION Phenylacetate can decrease the expression of ADAR2 mRNA in glioma cells, suggesting that phenylacetate, as a drug, may act on the course of RNA editing in gliomas.