RT Journal Article
SR Electronic
T1 The Influence of Different Single Radiation Dose on Delayed Growth of Transplanted Tumor in Athymic Mouse
JF Clinical Oncology and Cancer Research
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 303
OP 309
DO 10.1007/s11805-010-0536-6
VO 7
IS 5
A1 Zhi-zhen WANG
A1 Zhi-yong YUAN
A1 Ping WANG
YR 2010
UL http://www.cancerbiomed.org/content/7/5/303.abstract
AB OBJECTIVE To reveal the biological effects and effective dosage in radiotherapy model which applies high single-dose irradiation by animal experiment.METHODS We inoculated subcutaneouly human pancreatic carcinoma cell line (MIA PaCa-2) in the lateral of the right lower extremity of the athymic mouse to grow transplantation tumor. While the median diameter of transplantation tumor reached 10 mm approximately, the animals were randomly divided into 7 groups (6 animals per group) and fixed with consciousness for irradiation by different dose in one fraction (0, 2, 5, 10, 17, 25, 35 Gy). All were kept on to be bred for observation of the change in gross tumor volume, calculation of delayed growth time and delayed growth curve.RESULTS With increased dose per fraction, cutaneous reaction on the neoplasma surface of the animal, which was mainly moist yellow effusion was more and more severe. When dosage is less than 10 Gy, all animals showed similar effects, that’s the delayed tumor growth was not obvious. Tumors receiving more than 10 Gy in one fraction showed very good biological effect and the delayed tumor growth was obviously related to dosage. The difference in delayed tumor growth between the 2 groups was statistically significant. The delayed tumor growth time in 10, 17, 25 Gy group was respectively 3 weeks, 6 weeks and more.CONCLUSION The biological effect of the model which applies high single-dose irradiation (more than 10 Gy in one fraction) was very good. The effect of delayed tumor growth was obviously related to the dosage after transplantation tumor was radiated. Because of its higher dose per fraction and biological effects, the model of high single-dose irradiation can get better clinical effects.