PT - JOURNAL ARTICLE AU - Hua YANG AU - Jian-zhi ZHANG AU - Cheng-chao SHOU TI - Detection of Mycoplasma Hyorhinis Infection in Ovarian Cancer with <em>in situ</em> Hybridization and Immunohistochemistry AID - 10.1007/s11805-010-0534-8 DP - 2010 Oct 01 TA - Clinical Oncology and Cancer Research PG - 294--298 VI - 7 IP - 5 4099 - http://www.cancerbiomed.org/content/7/5/294.short 4100 - http://www.cancerbiomed.org/content/7/5/294.full SO - Cancer Biol Med2010 Oct 01; 7 AB - OBJECTIVE To detect Mycoplasma hyorhinis in ovarian cancer tissues and the relationship between mycoplasma infection and ovarian cancer.METHODS All specimens obtained from 109 cases with ovarian cancer were fixed in freshly prepared 10% neutral buffered formalin, embedded in paraffin, and cut into 4-μm sections for insitu hybridization (ISH) and then detected with immunohistochemistry (IHC). The expressions of 16S rRNA and P37 protein from mycoplasma hyorhinis were detected respectively using ISH and IHC. SPSS 13.0 software was employed to analyze the relationship between the results of the study and clinical pathological materials.RESULTS The expression rate of mycoplasma hyorhinis 16S rRNA gene and P37 protein was 20.2% (22/109) and 43.1% (47/109 cases) in ovarian cancer tissues, respectively, but it was 0 (0/30 cases) in the normal ovarian tissues. The difference in mycoplasma infection ratio between ovarian cancer tissues and normal tissues was extremely significant (P &lt; 0.001). Anyhow, we didn’t found any association between the mycoplasma infection and clinical pathological characters.CONCLUSION There was a mycoplasma infection in ovarian cancer tissues, which may play a role in oncogenesis of ovarian cancer.