RT Journal Article
SR Electronic
T1 Analysis of Up-Regulation of DNA-PKcs and Its Mechanism in Human Gliomas
JF Clinical Oncology and Cancer Research
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 122
OP 127
DO 10.1007/s11805-010-0506-z
VO 7
IS 2
A1 Zhi-xiang ZHUANG
A1 Li-qin SHEN
A1 Shu-yu ZHANG
A1 Peng QIU
YR 2010
UL http://www.cancerbiomed.org/content/7/2/122.abstract
AB OBJECTIVE To detect the differences in gene expression of nonhomologous end-joining pathway including Ku70, Ku80, ERCC4, lig4 and DNA-PKcs between human primary gliomas and normal brain tissues, and furthermore, to explore the underlying mechanism for the expression alteration.METHODS The expression levels of Ku70, Ku80, ERCC4, lig4 and DNA-PKcs in 36 specimens of glioma and 12 specimens of normal brain tissue were measured using SYBR green-based real-time quantitative PCR. Methylation of DNA-PKcs was detected through methylation-specific PCR (MSP).RESULTS There was no significant difference in expression of Ku70, Ku80, ERCC4 and lig4 between human primary gliomas and normal brain tissues (P < 0.05), while DNA-PKcs were significantly up-regulated (P = 0.002). The expression of DNA-PKcs was significantly higher in patients with grade III and IV diseases compared to patients with grade II disease or in normal brain tissues (P < 0.05). Moreover, glioma tissue showed weaker methylation than normal brain tissue.CONCLUSION The up-regulation of the DNA-PKcs may be associated with pathogenesis of glioma. Demethylation of DNA-PKcs promoter is an important reason for its up-regulation.