PT  - JOURNAL ARTICLE
AU  - Zhi-xiang ZHUANG
AU  - Li-qin SHEN
AU  - Shu-yu ZHANG
AU  - Peng QIU
TI  - Analysis of Up-Regulation of DNA-PKcs and Its Mechanism in Human Gliomas
AID  - 10.1007/s11805-010-0506-z
DP  - 2010 Apr 01
TA  - Clinical Oncology and Cancer Research
PG  - 122--127
VI  - 7
IP  - 2
4099  - http://www.cancerbiomed.org/content/7/2/122.short
4100  - http://www.cancerbiomed.org/content/7/2/122.full
SO  - Cancer Biol Med2010 Apr 01; 7
AB  - OBJECTIVE To detect the differences in gene expression of nonhomologous end-joining pathway including Ku70, Ku80, ERCC4, lig4 and DNA-PKcs between human primary gliomas and normal brain tissues, and furthermore, to explore the underlying mechanism for the expression alteration.METHODS The expression levels of Ku70, Ku80, ERCC4, lig4 and DNA-PKcs in 36 specimens of glioma and 12 specimens of normal brain tissue were measured using SYBR green-based real-time quantitative PCR. Methylation of DNA-PKcs was detected through methylation-specific PCR (MSP).RESULTS There was no significant difference in expression of Ku70, Ku80, ERCC4 and lig4 between human primary gliomas and normal brain tissues (P < 0.05), while DNA-PKcs were significantly up-regulated (P = 0.002). The expression of DNA-PKcs was significantly higher in patients with grade III and IV diseases compared to patients with grade II disease or in normal brain tissues (P < 0.05). Moreover, glioma tissue showed weaker methylation than normal brain tissue.CONCLUSION The up-regulation of the DNA-PKcs may be associated with pathogenesis of glioma. Demethylation of DNA-PKcs promoter is an important reason for its up-regulation.