RT Journal Article
SR Electronic
T1 Clinical Significance of Telomerase Activity and Human Telomerase Reverse Transcriptase mRNA Expression for Differential Diagnosis of Malignant and Benign Liver Lesions
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 442
OP 447
VO 1
IS 6
A1 Fan, Ruifang
A1 Wang, Weifeng
A1 Bu, Xinxin
A1 Guo, Xianling
A1 Jia, Fengqi
A1 Li, Zhengyou
A1 Wu, Mengchao
A1 Wei, Lixin
YR 2004
UL http://www.cancerbiomed.org/content/1/6/442.abstract
AB OBJECTIVE To study the clinical significance of telomerase activity and human telomerase reverse transcriptase (hTERT) mRNA expression for differential diagnosis of malignant and benign liver lesions.METHODS Telomerase activity was determined by an ELISA-based telomeric repeat amplification protocol (ELISA-TRAP) assay on 130 surgical resected liver samples and 58 percutaneous biopsied liver samples. In addition, the samples were assayed for expression of hTERT mRNA measured by a reverse transcriptase-polymerase chain reaction (RT-PCR). Postoperative pathological examinations were also performed on these samples.RESULTS Among the 130 surgical liver samples, the positive rates of telomerase activity in hepatocellular carcinoma (HCC), liver cirrhosis and chronic hepatitis tissues were 85.9% (55/64), 25.0% (8/32) and 8.3% (2/24) respectively, and the positive rates of hTERT mRNA expression were 89.1% (57/64), 25.0% (8/32) and 8.3% (2/24) respectively. Neither telomerase activity nor hTERT mRNA expression was detected in 10 normal liver tissues. Among the 58 biopsied liver specimens, the positive rates of telomerase activity in HCC, cholangiocellular carcinoma, focal nodular hyperplasia, inflammatory pseudotumor and adenomatous hyperplasia tissues were 85.7% (30/35), 100% (4/4), 33.3% (4/12), 25.0% (1/4) and 33.3% (1/3) respectively, and the positive rates of hTERT mRNA expression were 88.6% (31/35), 100%(4/4), 33.3%(4/12), 25.0%(1/4) and 33.3%(1/3) respectively. The positive level of telomerase activity and hTERT mRNA expression in malignant liver tumors was significantly higher than that found in benign liver lesions (P<0.01).CONCLUSION Determination of telomerase activity or hTERT mRNA expression in percutaneous biopsied liver tissues may be useful for differential diagnosis in malignant and benign liver lesions.