RT Journal Article
SR Electronic
T1 Differential Expression of Motility-Related Protein-1 Gene in Gastric Cancer and Its Premalignant Lesions
JF Chinese Journal of Clinical Oncology
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 111
OP 115
VO 1
IS 2
A1 Yao Xu
A1 Jie Zheng
A1 Wentian Liu
A1 Jun Xing
A1 Yanyun Li
A1 Xin Geng
A1 Weiming Zhang
YR 2004
UL http://www.cancerbiomed.org/content/1/2/111.abstract
AB OBJECTIVE To identify genes related to gastric cancer and to analyze their expression profiles in different gastric tissues.METHODS The differentially expressed cDNA bands were assayed by fluorescent differential display from gastric cancer specimens, matched with normal gastric mucosa and premalignant lesions. The motility-related protein-1 (MRP-1/CD9) gene expression was studied by Northern blots and reverse transcription polymerase chain reaction (RT-PCR) in different kinds of gastric tissueRESULTS A differentially expressed cDNA fragment showed lower expression in all gastric cancers compared to the normal gastric mucosa and premalignant lesions: and it was found to be homologous to the MRP-1/ CD9 gene. Northern blot analysis confirmed the differential expression. RT-PCR analysis showed that the MRP-1/CD9 gene was expressed at a much lower rate in gastric cancers (0.31 ±0.18) compared to the matched normal gastric tissue (0.49±0.24) and premalignant lesions (0.47±0.18)(P<0.05). Furthermore, its expression in intestinal-type of gastric cancer (0.38±0.16) was higher than that expressed in a diffuse-type of gastric cancer (0.22± 0.17)(P<0.05).CONCLUSION The MRP-1/CD9 gene expression was down-regulated in gastric cancer and its expression may be related to the carcinogenic process and histological type of gastric cancer.