RT Journal Article
SR Electronic
T1 The Influence of IFN-α on Blood Plasmacytoid Dendritic Cell in Chronic Myeloid Leukaemia
JF Clinical Oncology and Cancer Research
JO Cancer Biol Med
FD China Anti-Cancer Association
SP 113
OP 116
DO 10.1007/s11805-009-0113-z
VO 6
IS 2
A1 Chongyang Wu
A1 Liansheng Zhang
A1 Ye Chai
A1 Feixue Song
A1 Pengyun Zeng
A1 Lijuan Li
A1 Lingling Yue
A1 Bin Xiong
YR 2009
UL http://www.cancerbiomed.org/content/6/2/113.abstract
AB OBJECTIVE To study the mechanism of IFN on CML.METHODS Samples of 15 CML patients and 10 healthy controls were studied. The flow cytometry was performed to identify circulating pDCs. The concentration of IFN-α in serum and that in the supernatant of peripheral blood mononuclear cells (PBMCs) cultured after stimulation with CpG ODN2216 were examined both in CML patients and in the healthy controlsRESULTS There was significant reduction in the number of circulating pDCs, serum concentration of IFN-α and the capacity of IFN-α producing PBMCs in CML patients compared with those in healthy control individuals (P < 0.001). After the active treatment with IFN-α and hydroxyurea, the quantity and function of pDCs were increased in stabilized patients, especially the function of pDCs in 2 patients achieving major cytogenetic response (MCR). The proportion and function of pDCs and the serum levels of IFN were inversely correlated with both WBC and age of the patients with CML, and positively correlated with the state of the illness.CONCLUSION CML patients had a reduced number and dysfunction of circulating pDCs. The active treatment with IFN in CML patients may be related to the restoration of pDCs.