PT  - JOURNAL ARTICLE
AU  - Chuangui Chen
AU  - Jianqiu Chen
AU  - Jinjin Sun
TI  - HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions
AID  - 10.1007/s11805-009-0010-5
DP  - 2009 Feb 01
TA  - Clinical Oncology and Cancer Research
PG  - 10--15
VI  - 6
IP  - 1
4099  - http://www.cancerbiomed.org/content/6/1/10.short
4100  - http://www.cancerbiomed.org/content/6/1/10.full
SO  - Cancer Biol Med2009 Feb 01; 6
AB  - OBJECTIVE To explore the role of hypoxic inducible factor-1α (HIF-1α) in the proliferation and apoptosis of pancreatic cancer cells under hypoxic conditions.METHODS A cassette encoding small interference RNA (siRNA) targeting HIF-1α mediated by recombinant adeno-associated virus (rAAV) was constructed, giving rAAV-siHIF. rAAV-siHIF or rAAV-hrGFP was transfected into exponentially growing MiaPaCa2 cells under hypoxic conditions. Then, the expression of HIF-1α mRNA and protein, the proliferation and apoptosis of MiaPaCa2 cells were examined, using real-time PCR, Western Blot, MTT and TUNEL, respectively.RESULTS Under hypoxic conditions, rAAV-siHIF inhibited the expression of HIF-1α mRNA and protein in MiaPaCa2 cells. At the same time, rAAV-siHIF decreased MiaPaCa2 cell proliferation and induced apoptosis. However, rAAV-hrGFP had no effect on the expression of HIF-1α as well as the proliferation and apoptosis of MiaPaCa2 cells under hypoxic conditions.CONCLUSION Under hypoxic conditions, HIF-1α plays a key role in the proliferation of MiaPaCa2 cells, and inhibition of HIF-1α expression can lead to MiaPaCa2 cell apoptosis.