<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Chen, Chuangui</style></author><author><style face="normal" font="default" size="100%">Chen, Jianqiu</style></author><author><style face="normal" font="default" size="100%">Sun, Jinjin</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Clinical Oncology and Cancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009-02-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">10-15</style></pages><doi><style  face="normal" font="default" size="100%">10.1007/s11805-009-0010-5</style></doi><volume><style face="normal" font="default" size="100%">6</style></volume><issue><style face="normal" font="default" size="100%">1</style></issue><abstract><style  face="normal" font="default" size="100%">OBJECTIVE To explore the role of hypoxic inducible factor-1α (HIF-1α) in the proliferation and apoptosis of pancreatic cancer cells under hypoxic conditions.METHODS A cassette encoding small interference RNA (siRNA) targeting HIF-1α mediated by recombinant adeno-associated virus (rAAV) was constructed, giving rAAV-siHIF. rAAV-siHIF or rAAV-hrGFP was transfected into exponentially growing MiaPaCa2 cells under hypoxic conditions. Then, the expression of HIF-1α mRNA and protein, the proliferation and apoptosis of MiaPaCa2 cells were examined, using real-time PCR, Western Blot, MTT and TUNEL, respectively.RESULTS Under hypoxic conditions, rAAV-siHIF inhibited the expression of HIF-1α mRNA and protein in MiaPaCa2 cells. At the same time, rAAV-siHIF decreased MiaPaCa2 cell proliferation and induced apoptosis. However, rAAV-hrGFP had no effect on the expression of HIF-1α as well as the proliferation and apoptosis of MiaPaCa2 cells under hypoxic conditions.CONCLUSION Under hypoxic conditions, HIF-1α plays a key role in the proliferation of MiaPaCa2 cells, and inhibition of HIF-1α expression can lead to MiaPaCa2 cell apoptosis.</style></abstract></record></records></xml>