PT - JOURNAL ARTICLE AU - Luebke, Andreas M. AU - Attarchi-Tehrani, Ali AU - Meiners, Jan AU - Hube-Magg, Claudia AU - Lang, Dagmar S. AU - Kluth, Martina AU - Tsourlakis, Maria Christina AU - Minner, Sarah AU - Simon, Ronald AU - Sauter, Guido AU - Büscheck, Franziska AU - Jacobsen, Frank AU - Hinsch, Andrea AU - Steurer, Stefan AU - Schlomm, Thorsten AU - Huland, Hartwig AU - Graefen, Markus AU - Haese, Alexander AU - Heinzer, Hans AU - Clauditz, Till S. AU - Burandt, Eike AU - Wilczak, Waldemar AU - Höflmayer, Doris TI - Loss of PSP94 expression is associated with early PSA recurrence and deteriorates outcome of <em>PTEN</em> deleted prostate cancers AID - 10.20892/j.issn.2095-3941.2018.0384 DP - 2019 May 01 TA - Cancer Biology and Medicine PG - 319--330 VI - 16 IP - 2 4099 - http://www.cancerbiomed.org/content/16/2/319.short 4100 - http://www.cancerbiomed.org/content/16/2/319.full SO - Cancer Biol Med2019 May 01; 16 AB - Objective Prostate secretory protein of 94 amino acids (PSP94) is a target gene of the EZH2 transcriptional repressor and is often downregulated in prostate cancer; however, its prognostic value is disputed.Methods Immunohistochemical analysis of a tissue microarray of 12, 432 prostate cancer specimens was performed to evaluate PSP94 expression. Correlation of PSP94 expression with tumor phenotype, patient prognosis, TMPRSS2:ERG fusion status, EZH2 expression and PTEN deletion was studied.Results PSP94 expression was increased in benign prostatic hyperplasia; however, it was downregulated in 48% and negative in 42% of the 9, 881 interpretable prostate cancer specimens. The loss of PSP94 expression was inversely correlated to EZH2 expression (P &lt; 0.0001) and largely unrelated to the ERG status, but strongly correlated with high Gleason grade, advanced tumor stage, and nodal metastasis ( P &lt;0.0001 each). The fraction of PSP94-negative cancer specimens increased from 40% in pT2 to 52% in pT3b-pT4 ( P &lt; 0.0001) and from 40% in Gleason 3+3 = 6 to 46% in Gleason 4+3 = 7 and 60% in Gleason ≥4+4 = 8 ( P &lt; 0.0001). Loss of PSP94 was linked to early prostate-specific antigen recurrence, but with little absolute effect ( P &lt; 0.0001). However, it provided additional prognostic impact in cancer specimens with PTEN deletion. Loss of PSP94 deteriorated prognosis of cancer patients with PTEN deletion by more than 10% (P &lt; 0.0001). The combination of PTEN deletion and PSP94 loss provided independent prognostic information that was observed in several subgroups defined by classical and quantitative Gleason grade.Conclusions The results of our study suggest that combined PSP94/PTEN analysis can be potentially used in the clinical prognosis of prostate cancer.