Single-cell RNA sequencing to explore immune cell heterogeneity
E Papalexi, R Satija - Nature Reviews Immunology, 2018 - nature.com
Advances in single-cell RNA sequencing (scRNA-seq) have allowed for comprehensive
analysis of the immune system. In this Review, we briefly describe the available scRNA-seq …
analysis of the immune system. In this Review, we briefly describe the available scRNA-seq …
Antibiotics degradation by advanced oxidation process (AOPs): Recent advances in ecotoxicity and antibiotic-resistance genes induction of degradation products
Antibiotic contamination could cause serious risks of ecotoxicity and resistance gene
induction. Advanced oxidation processes (AOPs) such as Fenton, photocatalysis, activated …
induction. Advanced oxidation processes (AOPs) such as Fenton, photocatalysis, activated …
[BOOK][B] Environmental DNA: For biodiversity research and monitoring
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …
samples (such as soil, water, feces, or air) without the prior isolation of any target organism …
Third-generation in situ hybridization chain reaction: multiplexed, quantitative, sensitive, versatile, robust
HMT Choi, M Schwarzkopf, ME Fornace… - …, 2018 - journals.biologists.com
In situ hybridization based on the mechanism of the hybridization chain reaction (HCR) has
addressed multi-decade challenges that impeded imaging of mRNA expression in diverse …
addressed multi-decade challenges that impeded imaging of mRNA expression in diverse …
Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method
KJ Livak, TD Schmittgen - methods, 2001 - Elsevier
The two most commonly used methods to analyze data from real-time, quantitative PCR
experiments are absolute quantification and relative quantification. Absolute quantification …
experiments are absolute quantification and relative quantification. Absolute quantification …
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
Background Gene-expression analysis is increasingly important in biological research, with
real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high …
real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high …
Real-time quantification of microRNAs by stem–loop RT–PCR
C Chen, DA Ridzon, AJ Broomer, Z Zhou… - Nucleic acids …, 2005 - academic.oup.com
A novel microRNA (miRNA) quantification method has been developed using stem–loop RT
followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones …
followed by TaqMan PCR analysis. Stem–loop RT primers are better than conventional ones …
qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data
J Hellemans, G Mortier, A De Paepe, F Speleman… - Genome biology, 2007 - Springer
Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling
of selected genes, accurate and straightforward processing of the raw measurements …
of selected genes, accurate and straightforward processing of the raw measurements …
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
SA Bustin - Journal of molecular endocrinology, 2000 - jme.bioscientifica.com
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method
for the detection of low-abundance mRNA, often obtained from limited tissue samples …
for the detection of low-abundance mRNA, often obtained from limited tissue samples …
Comprehensive Identification of Cell Cycle–regulated Genes of the Yeast Saccharomyces cerevisiae by Microarray Hybridization
We sought to create a comprehensive catalog of yeast genes whose transcript levels vary
periodically within the cell cycle. To this end, we used DNA microarrays and samples from …
periodically within the cell cycle. To this end, we used DNA microarrays and samples from …
