We describe a simple method of using rolling circle amplification to amplify vector DNA such
as M13 or plasmid DNA from single colonies or plaques. Using random primers and φ29 …

We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–
7 kb in size by> 109-fold, using φ29 DNA polymerase. The principal difficulty with …

Amplification of source DNA is a nearly universal requirement for molecular biology
applications. The primary methods currently available to researchers are limited to in vivo …

We have developed a novel, isothermal DNA amplification strategy that employs⌽ 29 DNA
polymerase and rolling circle amplification to generate high-quality templates for DNA …

Techniques for the single-step amplification of whole genomes have been developed into
powerful tools for phylogenetic analyses, epidemiological studies and studies on genome …

We have developed a rapid method for sequencing supercoiled plasmid DNA and
bacteriophage λ DNA. The plasmid DNA is obtained from a 5-ml overnight culture using a …

ABSTRACT A method for obtaining sequence information directly from plasmid DNA is
presented. The procedure involves the rapid preparation of clean supercoiled plasmid DNA …

A strategy for rapid DNA sequence acquisition in an ordered, nonrandom manner, while
retaining all of the conveniences of the dideoxy method with M13 transducing phage DNA …

Quicker ways of sequencing short DNA would facilitate both mutational analyses and long
range mapping with sequence tagged sites (STS). The method of sequencing directly from …

We described previously a novel DNA amplification technique, termed ramification
amplification (RAM)(Zhang et al., Gene 211 (1998) 277). This method was designed to …