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Research ArticleOriginal Article

A New Method of Establishing Orthotopic Bladder Transplantable Tumor in Mice

Xi-hua Yang, Lian-sheng Ren, Guo-ping Wang, Li-li Zhao, Hong Zhang, Zhen-guo Mi and Xihua Bai
Cancer Biology & Medicine December 2012, 9 (4) 261-265; DOI: https://doi.org/10.7497/j.issn.2095-3941.2012.04.007
Xi-hua Yang
1Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China
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Lian-sheng Ren
1Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China
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  • For correspondence: [email protected]
Guo-ping Wang
1Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China
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Li-li Zhao
1Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China
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Hong Zhang
2Shanxi Institute for Food and Drug Control, Taiyuan 030001, China
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Zhen-guo Mi
1Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China
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Xihua Bai
1Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China
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  • Figure 1.
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    Figure 1.

    Schematic diagram of the pipe casing (A) and the drift angle stylet (B).

  • Figure 2.
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    Figure 2.

    Comparison between the mucosa injured and mucosa uninjured urinary bladder. A: mucosa uninjured; B: mucosa injured. The bladders were injected with physiological saline.

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    Figure 3.

    Comparison between the early-stage tumor and advanced-stage tumor on the urinary bladder. A: tumor formation in the early stage; B: tumor formation in the advanced stage.

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    Figure 4.

    Metastasis in the liver (A) and the kidney (B).

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    Figure 5.

    Survival time function of the T739 mice (A) and Balb/C-nu-nu nude mice (B).

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    Figure 6.

    Pathological images of different groups. A: The normal control group bladder (H&E staining, ×100); B: The model group bladder with tumor (H&E staining, ×100); C: The metastasis in liver (H&E staining, ×200); D: The metastasis in kidney (H&E staining, ×200).

Tables

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    Table 1.

    Comparison of tumor incidence between the mucosa uninjured bladder and mucosa injured bladder by drift angle stylet.

    GroupsNumber of miceCell inoculation amountTumor developed amountTumor incidence (%)
    T739 without injury101×10600
    T739 with injury601×10660100
    Balb/C-nu-nu without injury101×10600
    Balb/C-nu-nu with injury601×10660100
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    Table 2.

    Average survival time and bladder weight of the mice transplanted with tumor cells.

    GroupsNumber of miceAverage survival time (days)Average tumor wet weight
    T739 without injury10>400.02±0.22
    T739 with injury6026.69±9.240.54±0.37
    Balb/C-nu-nu without injury10>600.02±0.32
    Balb/C-nu-nu with injury6034.59±9.800.11±0.13
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Cancer Biology and Medicine: 9 (4)
Cancer Biology & Medicine
Vol. 9, Issue 4
1 Dec 2012
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A New Method of Establishing Orthotopic Bladder Transplantable Tumor in Mice
Xi-hua Yang, Lian-sheng Ren, Guo-ping Wang, Li-li Zhao, Hong Zhang, Zhen-guo Mi, Xihua Bai
Cancer Biology & Medicine Dec 2012, 9 (4) 261-265; DOI: 10.7497/j.issn.2095-3941.2012.04.007

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A New Method of Establishing Orthotopic Bladder Transplantable Tumor in Mice
Xi-hua Yang, Lian-sheng Ren, Guo-ping Wang, Li-li Zhao, Hong Zhang, Zhen-guo Mi, Xihua Bai
Cancer Biology & Medicine Dec 2012, 9 (4) 261-265; DOI: 10.7497/j.issn.2095-3941.2012.04.007
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