Research ArticleResearch Article

Influence of Vein Injury in Different Methods of Chemotherapy in Mice

Yong LI, Zhi GUO, Xiu-ying GUO, Zhe WANG and Wen-ge XING
Clinical Oncology and Cancer Research June 2011, 8 (2) 100-105; DOI: https://doi.org/10.1007/s11805-011-0566-8

Article Figures & Data

Figures

  • Fig. 1.
    Fig. 1.

    On the seventh day after cisplatin chemotherapy, a micro-quantity of brown granules could be seen staining the intima of the femoral vein of mice in the untreated group.

  • Fig. 2.
    Fig. 2.

    On the 14th day after cisplatin chemotherapy, a small quantity of brown granules could be seen staining the intima of the femoral vein of mice in the untreated group.

  • Fig. 3.
    Fig. 3.

    On the 14th day after cisplatin chemotherapy, a medium quantity of brown granules could be seen staining the intima of the femoral vein of mice in the intravenous chemotherapy group.

  • Fig. 4.
    Fig. 4.

    On the 21st day after cisplatin chemotherapy, a large quantity of brown granules could be seen staining the intima of the femoral vein of mice in the intravenous chemotherapy group.

  • Fig. 5.
    Fig. 5.

    On the 21st day after cisplatin chemotherapy, a large quantity of brown granules could be seen staining the intima of the femoral vein of mice in the untreated group.

  • Fig. 6.
    Fig. 6.

    Expression of granules stained for von Willebrand factor at the intima of the femoral vein.

  • Fig. 7.
    Fig. 7.

    On the third day after chemotherapy, the endothelial cells of the femoral vein of mice in the untreated group were continuously arrayed and tightly adhered to the basal membrane, the proportion of karyotypes was normal, the organelle morphosis was normal, and only a small quantity of synthesized granules was visible.

  • Fig. 8.
    Fig. 8.

    On the 14th day after cisplatin chemotherapy, the endothelial cells of the femoral vein of mice in the intravenous chemotherapy group had closely adhered to the basal membrane, but the cell surface was rough, the cells were in an activated state, the cells were hypertrophied, the cytoplasm was filled with various types of synthesized granules, and enlarged Weibel-Palade bodies could be seen in the cytoplasm.

  • Fig. 9.
    Fig. 9.

    On the 21st day after cisplatin chemotherapy, the endothelial cells of the femoral vein of mice in the intravenous chemotherapy group were not continuously arrayed, large quantities of endothelial cells were detached and separated from the basal membrane, the endothelial cells were in an activated state, the cell surface was rough, the cytoplasm was filled with synthesized granules, the proportion of karyotypes became unbalanced, and there was a significant increase in heterochromatins.

Tables

  • Table 1.

    Expression of granules stained for von Willebrand factor at the intima of the femoral vein.

    Group3rd Day7th Day14th Day21st Day28th Day
    Untreated0  8.82 +1.73*†14.59 + 2.40*†28.37+ 2.65*†28.86 + 1.98*
    Intravenous chemotherapy013.21 +2.11*†23.63+ 1.84*†36.17+3.68*37.27+3.72*
    Intraperitoneal chemotherapy012.78 +1.89*†21.53 + 3.81*†34.51 +2.12*†36.72 + 2.18*

    • ↵* Comparison of various groups at the same time points: statistically significant differences were found between the untreated group and the intravenous chemotherapy group (P < 0.05), and between the untreated group and the intraperitoneal chemotherapy group (P < 0.05), but no statistically significant differences were found between the intravenous chemotherapy group and the intraperitoneal chemotherapy group (P > 0.05).

    • ↵† Statistically significant differences were found between different time points of the same group (P < 0.05).

  • Table 2.

    Expression of granules stained for von Willebrand factor at the intima of the femoral vein (electron microscope).

    Group3rd Day7th Day14th Day21st Day28th Day
    Untreated group0  8.66 + 2.2*†*†  13.6 +1.58*†*†27.52 + 2.25*†*†30.88 + 2.55**
    Intravenous chemotherapy012.89+ 1.96*†*†22.53+ 2.02*†*†36.16 + 2.56*†*†38.08+ 2.02**
    Intraperitoneal hemotherapy011.96 + 2.29*†*†23.06+ 1.88*†*†35.46 + 2.15*†*†37.22+ 2.36**

    • ↵* Comparison of various groups at the same time points: statistically significant differences were found between the untreated group and the intravenous chemotherapy group (P > 0.05), and between the untreated group and the intraperitoneal chemotherapy group (P > 0.05), but no statistically significant differences were found between the intravenous chemotherapy group and the intraperitoneal chemotherapy group (P < 0.05).

    • ↵† Statistically significant differences were found between different time points of the same group (P < 0.05).

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