Research ArticleResearch Article

Association of Interleukin-10 Gene Haplotype with Gastric Cancer in a Chinese Population

Jing-yuan CUI, Hong MA, He WANG and Feng-bo SUN
Clinical Oncology and Cancer Research August 2010, 7 (4) 234-239; DOI: https://doi.org/10.1007/s11805-010-0524-x

Article Figures & Data

Figures

  • Fig. 1.
    Fig. 1.

    PCR-RFLP assay of −1082A/G IL-10 polymorphism. Lanes 1, 2, 3, 5, 6 and 7 are the homozygous AA genotype, showing A allele cut with EcoNI generating 280- and 97-bp fragments. Lanes 4 and 8 are the heterozygous AG genotype, with A allele cut with EcoNI generating 280- and 97-bp fragments, meanwhile, G allele cut with EcoNI generating 253-, 97- and 27-bp (not shown) fragments. Lane M is the marker.

  • Fig. 2.
    Fig. 2.

    PCR-RFLP assay of −819T/C IL-10 polymorphism. Lane 4 is the homozygous CC genotype, showing C allele cut with Mae III generating 125- and 84-bp fragments. Lanes 1, 6 and 8 are the heterozygous CT genotype, with C allele cut with Mae III generating 125- and 84-bp fragments, while T allele does not cut. Lanes 2, 3, 5 and 7 are the homozygous TT genotype, T allele does not cut, only 209-bp fragment. Lane M is the marker.

  • Fig. 3.
    Fig. 3.

    PCR-RFLP assay of −592A/C IL-10 polymorphism. Lanes 2, 4, 5 and 6 are the homozygous AA genotype, showing A allele cut with Rsa I generating 176- and 236-bp fragments. Lanes 3, 7 and 8 are the heterozygous AC genotype, with A allele cut with Rsa I generating 176- and 236-bp fragments; while C allele does not cut. Lane 1 is the homozygous CC genotype, C allele does not cut, only 412-bp fragment. Lane M is the marker.

  • Fig. 4.
    Fig. 4.

    The association between IL-10 gene polymorphisms and serum IL-10 levels.

Tables

  • Table 1.

    Clinical characteristics of the patients with gastric cancer and control group.

    Control (n = 180)GC (n = 220)P
    Gender (M/F)94/86134/860.081b
    Age (years)54.7 ± 14.0a57.3 ± 13.3a0.063c
    H. pylori infection positive (%)116 (64.4)147 (66.8)0.619b

    • ↵a Mean ± SD (all such values);

    • ↵b Pearson chi-square test;

    • ↵c 2-sample t-test.

  • Table 2.

    The genotype and allele frequencies of IL-10 polymorphism in patients with gastric cancer and control group.

    PolymorphismControl n = 180 (%)GC n = 220 (%)χ2P
    IL-10-1082A/G
        AA156 (86.7)171 (77.7)5.3030.021
        AG24 (13.3)49 (22.3)
        A allele336 (93.3)391 (88.9)4.7700.029
        G allele24 (6.7)49 (11.1)
    IL-10-819T/C
        TT80 (44.4)90 (40.9)6.4790.039
        CT87 (48.3)96 (43.6)
        CC13 (7.2)34 (15.5)
        T allele247 (68.6)276 (62.7)3.0280.082
        C allele113 (31.4)164 (37.3)
    IL-10-592A/C
        AA78 (43.3)95 (43.2)2.3540.308
        AC88 (48.9)98 (44.5)
        CC14 (7.8)27 (12.3)
        A allele244 (67.8)288 (65.5)0.4800.489
        C allele116 (32.2)152 (34.5)
  • Table 3.

    IL-10 gene haplotype distribution in the patients with gastric cancer and control group.

    HaplotypeControl 2n = 360 (%)GC 2n = 440 (%)Odds ratio (95% CI)P
    ATA238 (66.1)273 (62.0)1.00
    ACC83 (23.0)100 (22.7)1.01 (0.71-1.44)0.96
    GCC24 (6.7)49 (11.1)1.90 (1.11-3.27)0.02
    ACA6 (1.7)15 (3.4)2.06 (0.82-5.14)0.12
    ATC9 (2.6)3 (0.7)0.49 (0.17-1.41)0.19
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