Article Figures & Data
Figures
- Fig. 1.
The standard curve of FQ-PCR of MTA1 mRNA.
- Fig. 2.
Amplification map of MTA1 gene.
- Fig. 3.
Dissociation curve of MTA1 gene.
- Fig. 4.
The expression rate of MTA1 gene in the matched normal breast tissues.
Tables
Reagent Quantity (μl) Concentration ddH2O 5.285 10 X buffer 1.5 1 × dNTPs mixture (2.5 mM) 1.125 200 μM Mg2+ 0.9 2.5 mM Eva Green 0.75 Fluorescence 0.09 Taq DNA polymerase (2.5 μ/μl) 0.15 0.025 μ/μl Primer R (3 μM) 0.6 0.12 μM Primer F (3 μM) 0.6 0.12 μM Template (1:40) 4.0 Total volume 15.0 Gene n Cancer (dCt) Normal (dCt) Normal-cancer (ddCt) P MTA1 56 15.00 ± 4.39 17.58 ± 3.65 2.58 ± 4.82 0.032* dCt, the equation of the value of Ct of the measured gene and the gene of internal control; ddCt, the equation of the value of dCt of normal tissues and breast cancer.
↵* The difference between the two groups in the expression of MTA1 gene was statistically significant (P < 0.05).
- Table 3.
Relationship between the level of MTA1 mRNA in breast cancer and various clinicalpathologjc features in 56 cases with breast cancer.
Characteristics n MTA1 mRNA level mean ± SD P Lymph node metastasis Postive 37 1.52 ±2.53 0.001** Negative 19 4.64 ±2.12 Clinical grade I-II 40 1.98 ± 2.68 0.035* III-IV 16 4.08 ± 2.87 Up-inner 17 2.02 ± 3.51 Location Up-outer 12 2.87 ± 2.35 0.342 Down-inner 16 2.78 ± 2.98 Down-outer 11 2.84 ± 2.29 I 21 1.86 ± 2.68 Pathology grade II 23 2.42 ± 2.68 0.029* III 12 4.15 ± 2.87 < 2 26 1.97 ± 3.53 Tumor size (cm) 2-5 16 2.05 ± 2.55 0.042* > 5 14 4.32 ± 3.19 35- 11 2.58 ± 3.47 Age (years) 50- 30 2.51 ± 2.95 0.421 65- 15 2.72 ± 3.53 The difference between the expression level of the MTA1 gene and each group of different tumor size, clinical stage and pathologic grade was statistically significant.
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