Research ArticleResearch Article

Expression of Mitochondrial Transcripts in Gastric MGC803 Cell Line Subjected by Hypoxia

Chengbo Han, Jietao Ma and Huawei Zhou
Clinical Oncology and Cancer Research April 2009, 6 (2) 90-94; DOI: https://doi.org/10.1007/s11805-009-0090-2

Article Figures & Data

Figures

  • Fig. 1.
    Fig. 1.

    A, PAGE results of RT-PCR amplifying ND4 in hypoxic MGC803 cell lines. C1-C5: represent hypoxic time of 0, 2, 8, 16 and 24 h respectively M: pGEM-7Zf Marker (HuaMei Corporation). B, PAGE results of RT-PCR amplifying COI in hypoxic MGC803 cell lines. C, Trendgram of influence of hypoxia on mitochondrial transcripts in MGC803 cell lines. COI: cytochrome oxidase subunit I; ND4: NADH dehydrogenase subunit 4; ATPase6: ATPase subunit 6; cyt-b: cytochrome b.

  • Fig. 2.
    Fig. 2.

    Trendgram of COI and ND4 expression under hypoxia and cell cycle blockage under radiation after hypoxia in MGC803 cell lines

  • Fig. 3.
    Fig. 3.

    Trendgram of COI and ND4 expression under hypoxia and survival rate under radiation after hypoxia in MGC803 cell lines

Tables

  • Table 1.

    Primer pairs used for PCR-amplified mtRNA

    GenePrimerSequenceLength (mer)Tm (°C)GC%Product size (bp)
    COIF6043TCTAGGTAACGACCACATCTACAAC2562.444.0614
    R6656CGAAGCCTGGTAGGATAA1851.950.0
    ATPase6F9000CGCCTAACCGCTAACATTACTG2264.250.0148
    R9147AGGCGACAGCGATTTCTA1853.850.0
    ND4F11581ATCTGCCTACGACAAACA1848.344.4443
    R12024GTGGTGGGTGAGTGAGCCC1961.368.4
    ND5F13028CTGACTCCCCTCAGCCATAGA2157.257.1276
    R13303TGTGGTTGGTTGATGCCG1853.355.6
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