The Comparison of Biologic Characteristics between Mice Embryonic Stem Cells and Bone Marrow Derived Dendritic Cells

The margin of a colony of differentiated cell types derived from a single EB showing the early appearance of immature ESC-DCs as a “halo” of phase bright cells (A, 30×). The immature ESC-DCs had no characteristic veils of cytoplasm (B, 100×). When it was induced mature by adding LPS, the dendrites of ESC-DCs became apparent, and formed clusters (C, 200×). Immature BM-DCs had a few irregular dendrites (D, 200×), and were more distinct when becoming mature (E, 200×). 10~20 cell conglomerate could be seen (F, 200×). By electro-microscopy, the dramatic veils of cytoplasm and extensive dendrites of both ESC-DCs (G, 4000×) and BM-DCs (H, 4000×) could be seen clearly.