Article Figures & Data
Figures
- Fig.1.
Selection of cells with high CXCR4 expression by Real-time PCR A, GAPDH/T47D; B, CXCR4/T47D; C, GAPDH/MCF-7; D, CXCR4/MCF-7.
The values in the parentheses are Ct of amplification.
- Fig.2.
Restriction enzyme digest by BamH I and Hind III 1. siRNA1; 2. siRNA2; 3. Maker DL15000; 4. siRNA3; 5.Marker DL2000.
- Fig.3.
Flow cytometry evaluation of CXCR4 expression.
- Fig.4.
Cell-colony formation assay.
- Fig.5.
Matrigel invasion-chamber assay (40×).
- Fig.6.
The expression of tumor metastatic-associated genes.
Tables
Gene Primer sequences Fragment lengths (bp) CXCR4 5’-CCTATGCAAGGCAGTCCATGT-3’ 5’-GGTAGCGGTCCAGACTGATGA-3’ 86 GAPDH 5’-CATGAGAAGTATGACAACAGCCT-3’ 5’-AGTCCTTCCACGATACCAAAGT-3’ 113 Sequence Changed into siRNA templates siRNA1 AACCCTGTTTCCGTGAAGAAAAT 5’-GATCCGCCCTGTTTCCGTGAAGAAATTCAAGAGATTTCTTCACGGAAA
CAGGGTTTTTTGGAAA-3’siRNA2 AAGGGTGTGAGTTTGAGAACACT 5’-GATCCGGGTGTGAGTTTGAGAACATTCAAGAGATGTTCTCAAACTCA
CACCCTTTTTTGGAAA-3’siRNA3 AAGCGAGGTGGACATTCATCTGT 5’-GATCCGCGAGGTGGACATTCATCTTTCAAGAGAAGATGAATGTCCACCTC
GCTTTTTTGGAAA-3’The type of cells Ct(CXCR4) Ct(GAPDH) ΔCt ΔΔCt 2-ΔΔCt Inhibition rate (%) Control-siRNA 27.29 20.16 7.13 0 1 0 CXCR4-siRNA1 34.32 22.67 11.65 4.52 0.043285 95.7 CXCR4-siRNA2 32.12 22.16 9.96 2.83 0.140632 85.9 CXCR4-siRNA3 35.12 22.15 12.97 5.84 0.017458 98.3
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