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Research ArticleResearch Article

The Effect of Twist Expression on Angiogenesis in Hepatocellular Carcinoma

Gangmin Xi, Lin Zhang, Zhongli Zhan, Lihua Zhang, Xiyin Wei, Yi Yang, Yurong Shi, Fei Zhang and Ruifang Niu
Chinese Journal of Clinical Oncology December 2006, 3 (6) 413-418;
Gangmin Xi
1 Central Laboratory of Oncology,Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Lin Zhang
1 Central Laboratory of Oncology,Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Zhongli Zhan
2 Department of Pathology, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Lihua Zhang
2 Department of Pathology, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Xiyin Wei
1 Central Laboratory of Oncology, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Yi Yang
1 Central Laboratory of Oncology, Department of Pathology, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Yurong Shi
1 Central Laboratory of Oncology, Department of Pathology, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Fei Zhang
1 Central Laboratory of Oncology, Department of Pathology, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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Ruifang Niu
1 Central Laboratory of Oncology, Department of Pathology, Tianjin Medical University Cancer Institute & Hospital, Tianjin 300060, China.
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  • For correspondence: chenchy{at}mail.ihep.ac.cn
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  • Intensive staining of microvessels by anti-CD34 immunostaining (brownish staining) in a tumor section (A).Twist was highly expressed in the same patient(B).Staining of VEGF was also strong in the same patient(C). Photos were taken under 200⨯ magnifications.
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    Fig. 1.

    Intensive staining of microvessels by anti-CD34 immunostaining (brownish staining) in a tumor section (A).Twist was highly expressed in the same patient(B).Staining of VEGF was also strong in the same patient(C). Photos were taken under 200⨯ magnifications.

  • Positive correlation between Twist and VEGF expression scorings in HCC tissue samples was observed by using the Spearman rank correlation coefficient.
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    Fig. 2.

    Positive correlation between Twist and VEGF expression scorings in HCC tissue samples was observed by using the Spearman rank correlation coefficient.

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    Table 1.

    The relationship between Twist,VEGF,MVD and clinicopathological features

    TWISTVEGF
    CharacteristicsTotalNegativePositivep*NegativePositivep*MVDP**
    Gender
    Male341519122274.36 ± 30.86
    Female6330.789330.49381.72 ± 19.410.579
    Tumor size (cm)
    ≤5189961285.88 ± 25.98
    >5229130.5659130.62266.59 ± 29.640.037
    AFP level (μg/L)
    ≤2015787872.73 ± 26.64
    >202511140.8708170.35477.22 ± 31.290.647
    Tumor grade
    Well11654778.96 ± 32.97
    Moderate1991061380.26 ± 28.56
    Poor10370.507550.62061.04 ± 24.760.245
    Intrahepatic metastasis
    Present1851351382.58 ± 29.30
    Absent221390.04810120.25168.03 ± 28.160.123
    • ↵* P using χ2 test;

    • ↵** p: using independent-samples ttest or one-way ANOVA.

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Cancer Biology and Medicine: 3 (6)
Chinese Journal of Clinical Oncology
Vol. 3, Issue 6
1 Dec 2006
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The Effect of Twist Expression on Angiogenesis in Hepatocellular Carcinoma
Gangmin Xi, Lin Zhang, Zhongli Zhan, Lihua Zhang, Xiyin Wei, Yi Yang, Yurong Shi, Fei Zhang, Ruifang Niu
Chinese Journal of Clinical Oncology Dec 2006, 3 (6) 413-418;

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The Effect of Twist Expression on Angiogenesis in Hepatocellular Carcinoma
Gangmin Xi, Lin Zhang, Zhongli Zhan, Lihua Zhang, Xiyin Wei, Yi Yang, Yurong Shi, Fei Zhang, Ruifang Niu
Chinese Journal of Clinical Oncology Dec 2006, 3 (6) 413-418;
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