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Research ArticleResearch Article

An Inhibitory Effect of MAD1 on Bladder Tumor Cellular Proliferation in Vivo

Hongbo Hu, Chunli Luo, Xiaozhong Cai, Lin Zou, Pei Zhao and Xiouhou Wu
Chinese Journal of Clinical Oncology April 2006, 3 (2) 121-125;
Hongbo Hu
Department of Laboratory, The Third People’s Hospital of Liuzhou, Liuzhou Guangxi 545007, China.
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Chunli Luo
Department of Laboratory, Chongqing University of Medical Science, Chongqing 400016, China.
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  • For correspondence: luochunli79{at}hotmail.com
Xiaozhong Cai
Department of Laboratory, Chongqing University of Medical Science, Chongqing 400016, China.
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Lin Zou
Department of Laboratory, Chongqing University of Medical Science, Chongqing 400016, China.
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Pei Zhao
Department of Laboratory, Chongqing University of Medical Science, Chongqing 400016, China.
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Xiouhou Wu
Department of Urology, the First Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400016, China.
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    Fig. 1.

    Comparsion cell cycles of different groups (%, x;±s). *P<0.01 vs. groups B and C, **P>0.05 vs. groups B and C, ***P>0.05 vs. group C.

  • Fig. 2A.
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    Fig. 2A.

    Madl mRNA expression in different groups after transfection. 1: group A; 2: group B; 3: group C.

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    Fig. 2B.

    TERT mRNA expression in different groups after transfection. 1: group A; 2: group B; 3: group C.

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    Table 1.

    Primer Sequence and reaction parameters for different genes

    Gene NamePrimer SequenceProduct LengthReaction Parameters
    Madl

    sense: 5’-TGTGAGCGACTCTGACGA-3’

    antisense: 5’-GTGGGACACTGAAGTTTACG -3’

    210bp94”C,30 s; 54”C,30s; 72’C, 1 min
    β-actin

    sense: 5’-TTGTAACCTTCTGGGACGATATGG-3’

    antisense: 5’-TCATAGGCCCCGGTCACATC -3’

    650bp94-C.30 s; 55”C,30s: 72’C, 1 mm
    TERT

    sense: 5’-AGGTCATTCTTTTACATCAC-3’

    antisense: 5’-TCATAGGCCCCGGTCACATC -3’

    482bp94°C,30s; 53V, 30 s; 72V, 1 min
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    Table 2.

    Values of RBW, BAW and BRW for the different groups (n=ll, i±s)

    GroupRBW(g)BAW(mg)BRW (mg/g)
    A309 ± 14a302 ± 17b0.98 ± 0.07c
    B307+ 16d327+ 15d1.07 ± 0.08d
    C306 ± 14323 ± 161.06 ± 0.08
    • ↵a P>0.05 vs. group B and group C

    • ↵b P<0.01 vs. group B and group C

    • ↵c P<0.05 vs. group B and group C

    • ↵d P>0.05 vs. group C, BRW(mg/g)= BAW(mg)/RBW(g).

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Cancer Biology and Medicine: 3 (2)
Chinese Journal of Clinical Oncology
Vol. 3, Issue 2
1 Apr 2006
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An Inhibitory Effect of MAD1 on Bladder Tumor Cellular Proliferation in Vivo
Hongbo Hu, Chunli Luo, Xiaozhong Cai, Lin Zou, Pei Zhao, Xiouhou Wu
Chinese Journal of Clinical Oncology Apr 2006, 3 (2) 121-125;

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An Inhibitory Effect of MAD1 on Bladder Tumor Cellular Proliferation in Vivo
Hongbo Hu, Chunli Luo, Xiaozhong Cai, Lin Zou, Pei Zhao, Xiouhou Wu
Chinese Journal of Clinical Oncology Apr 2006, 3 (2) 121-125;
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Keywords

  • bladder tumor
  • animal model
  • cell cycle
  • transfection

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