Article Figures & Data
Figures
- Figure 1
Design, establishment, and quality control of the kinome-wide CRISPR-Cas9 library.
- Figure 2
Statistics of the CRISPR screening results.
- Figure 3
Representation of the top 10 significant genes.
- Figure 4
Pathway enrichment and protein-protein interaction network of the essential genes in osteosarcoma.
- Figure 5
Functional validation of TRRAP, PKMYT1, and TP53RK in osteosarcoma.
- Figure 6
TRRAP, PKMYT1, and TP53RK gene expression levels and clinical relevance in patients with osteosarcoma.
Tables
Component Amount Gibson Assembly Master Mix 10 μL Digested lenti-CRISPR V2 200 ng Purified oligonucleotide PCR product 20 ng Ultra-pure water To 20 mL Primers Sequence TRRAP-sg1-F CACCGGCGGCAGTCACCAGGTGCAG TRRAP-sg1-R AAACCTGCACCTGGTGACTGCCGCC TRRAP-sg2-F CACCGGAAGCAAGGAGAAAAGGACA TRRAP-sg2-R AAACTGTCCTTTTCTCCTTGCTTCC PKMYT1-sg1-F CACCGGGGCCATGGCTCCTACGGAG PKMYT1-sg1-R AAACCTCCGTAGGAGCCATGGCCCC PKMYT1-sg2-F CACCGAACATGGAGCTGCCCCACGG PKMYT1-sg2-R AAACCCGTGGGGCAGCTCCATGTTC TP53RK-sg1-F CACCGAGAGCTACTACGCCGGCCGA TP53RK-sg1-R AAACTCGGCCGGCGTAGTAGCTCTC TP53RK-sg2-F CACCGGCTGGTGAAGCAGGGTGCCG TP53RK-sg2-R AAACCGGCACCCTGCTTCACCAGCC Comparison Selection FDR 1% FDR 5% FDR 25% 143B-G16/G0 Negative 36 41 59 MG63-G16/G0 Negative 11 17 40 U2OS/MTX300-G16/G0 Negative 15 24 50 - Table 4
The top 10 significant genes whose knockout induced cell death in 143B, MG63, and U2OS-MTX300 cells
143B MG63 U2OS/MTX300 ID neg|score neg|P neg|fdr ID neg|score neg|P neg|fdr ID neg|score neg|P neg|fdr TRRAP 1.76E-10 9.75E-06 2.48E-04 PDGFRA 1.82E-10 9.75E-06 8.25E-04 TP53RK 5.44E-08 9.75E-06 9.90E-04 PKMYT1 6.80E-10 9.75E-06 2.48E-04 MAPK15 3.14E-10 9.75E-06 8.25E-04 PLK4 9.25E-08 9.75E-06 9.90E-04 RIOK1 1.14E-09 9.75E-06 2.48E-04 CDK6 1.58E-07 9.75E-06 8.25E-04 PLK1 1.98E-07 9.75E-06 9.90E-04 MTOR 2.66E-09 9.75E-06 2.48E-04 MAST1 2.96E-07 9.75E-06 8.25E-04 BRD2 5.22E-07 9.75E-06 9.90E-04 PLK1 4.32E-09 9.75E-06 2.48E-04 TP53RK 1.01E-06 9.75E-06 8.25E-04 GAK 3.68E-06 9.75E-06 9.90E-04 CDK2 9.89E-09 9.75E-06 2.48E-04 PTK2 1.64E-06 9.75E-06 8.25E-04 MTOR 1.01E-05 2.92E-05 1.65E-03 VRK1 1.58E-08 9.75E-06 2.48E-04 TRRAP 2.37E-05 1.66E-04 9.41E-03 TRRAP 1.10E-05 2.92E-05 1.65E-03 AURKB 3.73E-08 9.75E-06 2.48E-04 PKMYT1 2.53E-05 1.85E-04 9.41E-03 PKMYT1 1.19E-05 2.92E-05 1.65E-03 BUB1B 7.57E-08 9.75E-06 2.48E-04 ILK 2.69E-05 1.85E-04 9.41E-03 RIOK2 1.22E-05 2.92E-05 1.65E-03 CDK1 1.28E-07 9.75E-06 2.48E-04 NRBP2 2.92E-05 1.85E-04 9.41E-03 MAP3K9 1.71E-05 6.82E-05 3.47E-03
