Abstract
Sex-determining region Y box-containing genes are transcription factors with roles in multiple biological processes, including cell differentiation, proliferation, and apoptosis. Sex-determining region Y box-containing genes have also been shown to act as regulators and biomarkers in the progression of many different cancers, including gynecological cancers such as ovarian, cervical, and endometrial cancer. In this review, we summarize the contrasting regulatory roles of Sex-determining region Y box-containing genes in different gynecological cancers, as promotors with high expression levels or as suppressors with low expression levels. Expression levels of Sex-determining region Y box-containing genes were also identified as biomarkers of clinical features, including International Federation of Gynecology and Obstetrics stage, histopathologic grade together with disease-free survival, and treatment efficacy in patients with gynecological cancers. An understanding of the mechanisms whereby Sex-determining region Y box-containing genes regulate the progression of gynecological cancers will aid in the development of novel diagnostic and therapeutic strategies, while analysis of Sex-determining region Y box-containing expression levels will help to predict the prognosis of patients with gynecological cancers.
keywords
- Sex-determining region Y box-containing gene
- gynecological cancer
- regulator
- biomarker
- clinical feature
- progression
Introduction
Sex-determining region Y box-containing (SOX) genes encode regulatory transcription factors that can act as tumor suppressors or promoters in carcinogenesis. SOX genes were shown to be involved in the development of various cancers, including breast1, lung2, hepatocellular3, and gastrointestinal cancers4. Due to the increased morbidity and mortality of gynecological cancers (GCs) year by year, the roles of SOX genes in GCs, including ovarian (OC), cervical (CC), and endometrial cancer (EC) become a recent focus of researches5. Studies investigating the relationship between aberrant expression of SOX genes and the development of GCs found that some SOX genes with high expression level were expected to act as oncogenic regulators which promoting the progression of GCs, while those with low expression level were regarded as suppressors with the opposite effects6-11. Furthermore, regulating expression level of SOX genes in certain cancer cell lines could influence cell proliferation and apoptosis in vitro but without known mechanisms12,13. Additionally, analysis of abnormal SOX genes expression in patient samples contributed to the detection of early GC lesions14-16. Expression level of SOX genes was also considered as biomarkers associated with clinical features in patients with GCs, including International Federation of Gynecology and Obstetrics (FIGO) stage, histopathologic grade, and disease-free survival (DFS), as well as treatment response10,15,17-19. Understanding the mechanisms whereby SOX genes regulate the progression of GCs is therefore valuable and may facilitate the development of novel diagnostic, therapeutic, and prognostic strategies which aimed at improving the prognosis of women with GCs. This review provides a systematic summary of the SOX genes roles in the progression of GCs, and highlights future directions for research.
Classification and functions of SOX genes
Classification of SOX genes
SOX genes encode a conserved group of regulatory transcription factors comprising about 414-amino-acid polypeptides with a highly conserved high-mobility group (HMG) box20,21. This box encodes around 79-amino-acid DNA-binding domain, with two L-shaped arms that can bind to ATTGTT or related DNA sequence motifs in the minor groove by recognizing the sequence 5'-(A/T)(A/T)CAA(A/T)-3', resulting in widening of the minor groove, unwinding of the DNA helix, and DNA bending22. This domain was first identified in SRY, as a crucial factor involved in determining male sex of mammals. Genes that encode proteins containing an HMG domain with at least 50% amino acid similarity to the SRY HMG domain are considered as SOX genes23. To date, mammalian genomes have been found to include approximately 30 different SOX genes, which can be classified into 10 subgroups (AJ) based on the degree of homology of the amino acid sequence inside the HMG domain, the presence of conserved motifs outside the HMG domain, and their full-length structures (Figure 1)24-26. In this review, we summarize the roles of some GC-related SOX genes, including SOX1, SOX2, SOX3, SOX4, SOX7, SOX8, SOX9, SOX11, SOX14, SOX15, SOX17, and SOX18.
Classifications and structures of SOX families. (A) SOX genes contain approximately 30 different SOX genes which are classified into ten subgroups (AJ). These various groups are highlighted by different colors. Origins and connection between branches are based on the structures and function of SOX protein encoded by SOX genes. (B) Schematic representation of the structures of some known SOX protein. The HMG box, transactivation/repression domain and other functional domains are indicated along with the length of SOX proteins. Groups and representative protein members are indicated to the left. N-terminal and C-terminal domains of SRY are depicted at the top. The sizes in amino acids (aa) of the various SOX proteins are shown to the right.
Functions of SOX genes
The activities of the various subgroups of SOX genes are multi-faceted. Fundamentally, SOX genes are involved in sex determination and the development of the testis, prostate, endothelial cells, and the vascular, lymphatic, and nervous systems during vertebrate embryonic development24,27. However, the multiple functions of SOX genes in the development of these various systems alerted researchers to their potential roles in the development of diseases, especially cancers12. Most recent studies of SOX genes focused on their involvement in gastric cancer, lung cancer, hepatocellular carcinoma, and prostate cancer2-4,28,29. Studies indicated that most SOX genes played their roles in these cancers through the Wnt/ݭcatenin signaling pathway, as the so-called canonical Wnt pathway mediated by ݭcatenin12,13. Activation of the Wnt/ݭcatenin signaling pathway decreases phosphorylation of ݭcatenin in the cytoplasm and increases ݭcatenin transfer into the nucleus. Consequently, it activates the nuclear complex of ݭcatenin/T cell factor/lymphoid enhancer factors, and enhances expression level of cell cycle-related molecules such as cyclin-D1 and c-Myc12,30. Thus, a discussion of how SOX genes act in the progression of GCs via different signaling pathways, especially the Wnt/ݭcatenin signaling pathway is presented below.
SOX genes in GCs
Numerous studies currently focus on the roles of SOX genes in GCs. In this review, OC, CC, and EC are selected to be representative GCs due to their high incidence and mortality. In general, SOX genes have been identified as regulators influencing the progression of GCs, as well as biomarkers of clinical features. Clinical, cellular, and animal experiments have shown that some SOX genes act as oncogenes while others act as tumor suppressor genes in these three cancers (Figure 2). General depiction of the expression level of SOX genes in GCs and mechanisms of how they perform effectively are shown in Figure 3.
General summary of the researches on SOX genes in GCs. Darker blue coloration means more researches in the corresponding area as indicated in the legend on the right side of the heatmap. The number represents the corresponding number of researches. Onco: SOX that plays as an oncogene. Anti: SOX that plays as a tumor suppressor gene. Cell: researches based on cell lines. Patients: researches based on patients samples. Mice: researches based on nude mice.
Comprehensive depiction of the roles of SOX genes performed in GCs. SOX genes play different roles in different GCs. The black upward arrows indicate high expression level of SOX genes in the corresponding cancers, which play as promoters. The black down arrows indicate low expression of SOX genes the corresponding cancers, which play as inhibitors. The black circles indicate that the roles of SOX genes are controversial. The genes or molecules shown in the surrounding colored circles are among the pathways through which SOX genes play in GCs to promote cell proliferation, inhibit apoptosis, and enhance cell metastasis.
SOX genes in OC
OC is one of the three most common cancers in women, with an estimated 22,240 new diagnoses and 14,070 deaths in the United States in 20185. Due to lack of specific symptoms and reliable screening methods, approximately 70% of patients with OC are diagnosed at advanced stage with metastasis beyond the ovary, which contributes to its high mortality31,32. Thus, there is an urgent need to find novel diagnostic biomarkers for detecting OC at a premalignant stage33. SOX genes are identified as such biomarkers that can contribute to early screening and the prediction of clinical features in patients with OC, and regulation of SOX gene expression could influence cell proliferation and treatment efficacy at the cellular level9,10,16,18,31,34.
SOX genes as clinical biomarkers for OC
Regarding their roles as clinical biomarkers, multiple studies analyzed the relationships between SOX gene expression level in OC samples and clinical features, including FIGO stage, histopathologic grade, and DFS (Table 1). Researchers identified SOX1, SOX7, and SOX11 as tumor suppressor genes, with low expression level in patient samples due to aberrant CpG island hyper-methylation or unclear mechanisms17,18. Low expression level of these genes in cancerous tissues or serum was detected more frequently in patients with more advanced stage, higher grade, more aggressive tumor behavior, and shorter recurrence-free survival (RFS), while higher level was associated with the opposite clinical features10,17,18,35,40. These results suggested that analyzing SOX gene expression might be a good biomarker for predicting the prognosis of patients.
Abnormal expression of SOX genes and their potential clinical implications in gynecological cancers
However, in addition to tumor suppressor role of SOX genes, SOX2 was shown to play dual roles in OC, with high expression level identified as a poor prognostic biomarker in some cases, but as a favorable factor in other cases. On one hand, high SOX expression level in fallopian tube epithelium was exploited as a biomarker for OC screening, especially in BRCA1 or BRCA2 mutation carriers or in women with serous OC in high grade16. High expression level of SOX2 in patient samples was also shown to be related to high grade, advanced FIGO stage, and decreased DFS36,37. On the other hand, Pham et al.38 demonstrated that high expression level of SOX2 was a favorable biomarker indicating longer DFS and overall survival (OS) in patients with stage IIIV high-grade serous OC among 215 cases of OC. Other researchers also affirmed the good prognostic effects of SOX2 in 570 samples from patients with ovarian serous cystadenocarcinoma39. The mechanisms responsible for these different effects of SOX2 may be due to various factors, such as feedback mechanisms of SOX2 expression, interactions between SOX2 in the cytoplasm and nucleus, or differences between patient spectra and measuring methods. These flexible and bidirectional roles of SOX2 suggest that SOX2 could be a double-edged sword depending on how scientists choose to utilize it. However, analyzing expression level of SOX gene in tissues is still generally considered to be a valuable approach for predicting clinical features in patients with OC.
SOX genes as regulators in the progression of OC
In addition to their role as clinical biomarkers, accumulating evidence from in vitro studies indicated that SOX genes acted as vital regulators in the progression of OC, including in cell proliferation, apoptosis, and metastasis (Figure 4). SOX2 was considered as an oncogene at the cellular level, and up-regulation of SOX2 in OC cell lines promoted cell proliferation and tumor sphere formation via hypoxic treatment and overexpression of the intracellular domain of Notch9. Moreover, transduction of SOX2 into OC cell lines also enhanced resistance to cell apoptosis through overexpression of the anti-apoptotic gene BCL2 and simultaneous down-regulation of the pro-apoptotic genes PUMA/BBC3 and NOXA/PAMAIP134. Overexpression of SOX2 also accelerated cell migration by down-regulating E-cadherin and up-regulating vimentin expression31. These results at the cellular level were not as the same as the results based on clinical samples, which suggested that in vitro studies could not imitate the environment in the body completely. And more animal studies are needed to clarify the role of SOX2 in the progression of OC. In addition to SOX2, up-regulation of SOX4 in OC cell lines by the long non-coding RNA BRM promoted cell proliferation, migration, and invasion via an unknown mechanism62. More researches are therefore also needed to investigate the mechanisms and potential of SOX4 in OC.
Roles of SOX genes (SOX2, SOX4, SOX7 and SOX11) in the progression of OC. SOX2 and SOX4 performed as oncogenes in red color. SOX7 and SOX11 acted as tumor suppressor genes in blue color. Small green arrows indicate promotion and small red arrows indicate inhibition. Cyclin D1 and Cyclooxygenase2 (COX2) are promoting factors in the cell cycle. E-cadherin is an intercellular adhesion molecule, and vimentin is a protein that helps epithelial cells maintain characteristics of fibroblasts such as weaken adhesion and enhance mobility.
SOX7 and SOX11, which are considered as tumor suppressor genes at both the clinical and molecular level, inhibited the progression of OC. Low level of SOX7 together with increased cyclooxygenase-2 and cyclin-D1 were detected in tissues from patients with epithelial OC, especially in patients with advanced serous cystadenocarcinoma10. This suggested that SOX7 might regulate cell proliferation by influencing the Wnt/ݭcatenin signaling pathway. Transduction of SOX11 into OC cell lines was also reported to inhibit cell proliferation, though the mechanism remained unclear18. These members of the SOX gene family all contribute to the progression of OC, and their various mechanisms warrant more detailed investigation. These results also suggested that controlling the expression of certain SOX genes may be a promising therapeutic target in the near further.
SOX genes influence treatment efficacy in OC
In terms of treatment, overexpression of SOX2 was shown to enhance chemoresistance of OC cell lines to cisplatin through activating the expression of the drug efflux transporter genes ABCB1 and ABCG29. Knockdown of SOX2 in cell lines accordingly decreased chemoresistance to cisplatin via down-regulating expression of ABCB1, ABCG2, and ABCC631. These results were accordance with those of researches on the roles of SOX2 based on OC cell lines, but not with researches based on patients samples in other cases9,31,34,38,39. Further simultaneous in vitro and in vivo researches into SOX2 are therefore needed to develop its role as a promising new therapeutic target for patients with OC.
SOX genes in CC
CC is a common GCs considered to be an accidental endpoint of persistent infections with certain types of human papillomavirus (HPV), especially HPV16 and HPV185,63. Some investigators found that SOX2 regulated HPV16 transcription by inhibiting activity of its long control region and finally decreasing expression of the E6 and E7 oncogenes in CC carcinogenesis64. Furthermore, mounting evidence implicated other members of the SOX genes family in the regulation of CC progression (Figure 5) and identified their roles as biomarkers for predicting the prognosis of patients with CC (Table 1).
Roles of SOX genes (SOX1, SOX2, SOX4, SOX9, SOX14 and SOX18) in the progression of CC. Oncogenes included SOX4, SOX2 and SOX18 in red color. SOX1 was regarded as suppressor gene in blue color. SOX9 and SOX14 played bidirectional roles in purple color. Small green arrows indicate promotion and small red arrows indicate inhibition. There were few studies of SOX4 and SOX18 yet. P21WAF1/CIP1 is a main kind of cyclin-dependent kinase inhibitor in cell cycle.
SOX genes as screening biomarkers and prognostic factors in CC
Analysis of the aberrant expression level of SOX1,SOX8, SOX9, SOX14, and SOX17 were identified as a novel early screening method for distinguishing between early CC lesions and normal tissues by methylated-CpG island recovery assay (Table 1). Higher methylation level of these genes was accompanied by more severe cervical squamous cell lesions14,41-46,55. Moreover, the sensitivity and specificity of this early screening method was increased by the combined detection of the methylation level of more than one SOX gene, such as SOX1 and SOX14, or SOX8 and SOX1753,65. Further studies are therefore needed to determine if analyzing the expression level of all SOX genes combined might increase their sensitivity as screening biomarkers for CC.
In terms of prognostic biomarkers, SOX2 was identified as a dual-effect gene, with its expression level having different implications for clinical features in different studies. Four studies detected high level of SOX2 in tissues from CC patients and concluded that high SOX2 expression was associated with higher grade, poorer differentiation, advanced stage, and poorer survival7,48-50 (Table 1). However, the opposite effects were observed in other studies. For example, Kim et al.47 detected SOX2 expression in tissue samples from normal cervical epithelium, cervical intraepithelial neoplasia, and CC by immunohistochemistry, and found that high expression level of SOX2 were correlated with favorable DFS and OS. The unknown mechanisms behind this phenomenon may help to account for the roles of SOX2 in OC at the clinical level. Nevertheless, more researches are needed to elucidate the precise mechanisms responsible for the effects of SOX2.
SOX genes regulate progression of CC
Similar to their roles in OC, numerous studies investigated the involvement of SOX genes in regulating the progression of CC (Figure 5). Consistent with an oncogenic role, some studies found that transduction of CC cell lines with SOX2, SOX4, and SOX18 promoted cell proliferation, metastasis, and invasion52,66-68. Specifically, endogenous overexpression of SOX2 or SOX4 in CC cell lines drove the cell cycle from G0/G1 to S stage by promoting expression of cell cycle promoters, such as cyclinE2, minichromosome maintenance protein 10 (MCM10), and weel protein kinase52,66. Overexpression of SOX2 in CC cell lines also promoted metastasis and invasion by augmenting the expression of epithelialmesenchymal transition-promoted molecules, such as vimentin, ݭcatenin, and Snail67. However, the action of SOX2 at the cellular level did not necessarily reflect the same behavior in clinical samples because of the absence of the bodys internal environment. SOX18 promoted the development of CC without clear mechanisms, so further studies are needed to clarify the mechanisms responsible for the oncogenic role of SOX1868.
In contrast, SOX1 is considered as a tumor suppressor gene, in line with its activity in clinical samples as mentioned above. Up-regulation of SOX1 inhibited the growth of CC cells both in vitro and in vivo by impeding the transcriptional activity of T cell factor in the Wnt/ݭcatenin signaling pathway. It also promoted metastasis by up-regulating the cancer metastasis suppressor gene cadherin 1 (CDH1) and simultaneously down-regulating Snail2, the inhibitor of E-cadherin transcription6. Future researches should focus on exploring how SOX1 influence cell apoptosis and treatment efficacy.
Intriguingly, although SOX9 and SOX14 were regarded as tumor suppressor genes with low expression level detected in patients samples due to methylation, these two SOX genes presented dual functions based on research on cell lines. As oncogenes, some researchers reported that overexpression of SOX9 in CC cell lines promoted cell proliferation by down-regulating the expression of PTEN, which prevents cells from growing too quickly54. And overexpression of SOX14 were proved to boost cell proliferation and invasion by activating the Wnt/ݭcatenin signaling pathway along with high level of ݭcatenin69. In contrast, as tumor suppressor genes, up-regulation of SOX9 or SOX14 could block the cell cycle transition by activating the expression of p21WAF1/CIP1 and p53, resulting in suppression of cell growth and tumor formation in vitro. Overexpression of SOX14 also induced apoptosis by promoting the expression of Bax and cleaved-poly ADP-ribose polymerase8,70. These interesting results indicated the need for more thorough investigations to compare and combine the effects of different experimental conditions on outcomes.
Effects of SOX genes on treatment efficacy in CC
In terms of treatment, SOX2 and SOX4 are considered as oncogenes accordance with their roles in the progression of CC. Superficially, expression level of SOX2 was higher in tissues from patients with radiation-resistance compared with those with radiation-sensitivity, suggesting that SOX2 was a biomarker of unfavorable therapeutic reactivity51. Overexpression of SOX genes, such as SOX4, in Caski cell lines also decreased the treatment efficacy of cisplatin by up-regulating the drug efflux transporter gene ABCG252. And more investigations are required to explore how SOX genes influence treatment efficacy in patients with CC. In addition to SOX2 and SOX4, one study implied that high expression of SOX9 in CC cell lines enhanced cell resistance to cisplatin through combining with the promoter region of miR-130a and down-regulating expression of copper transporter protein 1 (CTR1), which is a significant factor affecting the activity of cisplatin54. However, this result was in contrast to the action of SOX9 in the progression of CC, and the function of SOX9 in the treatment of CC still needs clarification.
In conclusion, SOX genes play significant roles in CC, and SOX members may act as promising biomarkers or therapeutic targets in clinical practice in the near future.
SOX genes in EC
EC, with an estimated 63,230 new cases and approximately 11,350 deaths, was regarded as the most common female reproductive system malignancy in the United States in 20185. Despite extensive research focusing on exploring the genetic and epigenetic characteristics of EC, its pathogenesis and progression remain unclear. Recently, some studies indicated the important roles for SOX genes in regulating the progression of EC (Figure 6) and in indicating clinical features and treatment efficacy of patients with EC (Table 1)19,21,58,71,72.
Mechanisms of SOX genes (SOX2, SOX3, SOX4, SOX7, SOX9, SOX11, SOX15 and SOX17) in the development of EC. Oncogenes included SOX3, SOX4 and SOX11 in red color. Tumor suppressor genes consisted of SOX7, SOX15 and SOX17 in blue color. SOX2 and SOX9 played bidirectional roles in purple color. Small green arrows indicate promotion and small red arrows indicate inhibition. The mechanisms of SOX4, SOX11 and SOX15 have only been mentioned in a few researches yet. Akt promote growth factor-mediated proliferation and survival of cells both directly and indirectly. NF-κB is a protein complex that controlled transcription of DNA, cytokine production and cell survival. BCL2-associated X protein functions as an apoptotic activator. Cleaved caspase-3 is an executioner of apoptosis. Caspase-9 is an initiator of apoptosis. Survivin is an inhibitor of apoptosis. Mastermind like3 (MAML3) is a co-activator of ݭcatenin-mediated transcription.
SOX genes as biomarkers of clinical features in EC
Many researchers explored the roles of SOX genes in indicating the clinical features of patients with EC (Table 1). Low expression level of tumor suppressor SOX genes, such as SOX1, SOX7, SOX9, and SOX17, due to methylation and other mechanisms, could be considered as novel prognostic biomarkers of EC. For example, low expression level of SOX1, SOX7, and SOX17 was shown to be potential biomarkers for detecting EC masked by atypical hyperplasia, and indicated advanced stage, higher grade, and shorter RFS19,21,56,57,60. Additionally, high expression level of SOX17 in tissues indicated increased toxicity of cisplatin and high therapeutic sensitivity of patients61. However, SOX9 expression showed a significant stepwise increase from normal tissues through grade 1 to grade 2/3 cancer tissues, probably due to a hidden feedback system59. This study suggested that detecting the detailed mechanisms of SOX9 will provide valuable information.
Interestingly, SOX2 is identified as a bi-functional gene in EC, as in OC and CC. Pitynski et al.15 analyzed expression level of SOX2 in samples from EC patients and found higher expression level of it in high-grade (G3) compared with moderate-grade (G2) and low-grade (G1) of EC. High expression level of SOX2 in tissues was also associated with poorer outcomes of patients with advanced-stage EC11. In contrast, Wong et al.58 proposed that SOX2 was a tumor suppressor gene inhibiting the progression of EC, and low level of it was identified as an indicator of type II serous, clear cell adenocarcinoma as well as shorter survival. These phenomena suggested that further exploration of the molecular mechanisms of SOX2 should be carried out in relation to clinical management of EC.
SOX genes regulate progression of EC
In relation to the progression of EC, researchers regulated the expression of SOX gene in EC cell lines by transduction with the corresponding SOX genes. Through this method, they found that overexpression of SOX3, SOX4, and SOX11 promoted cell proliferation while overexpression of SOX7, SOX15, and SOX17 inhibited cell growth and accelerated apoptosis. Meanwhile, SOX2 and SOX9 were regarded as dual-function genes in the progression of EC (Figure 6).
As oncogenes, up-regulation of SOX3, SOX4, and SOX11 in EC cell lines was associated with accelerated cell proliferation via unknown mechanisms, while silencing of these genes had the inverse effects72-74. Moreover, SOX3 also promoted EC cell metastasis in vitro by down-regulating the epithelial marker E-cadherin and up-regulating the mesenchymal marker vimentin72. However, the roles of SOX4 and SOX11 in promoting cell metastasis and invasion remain unclear.
SOX7, SOX15, and SOX17, regarded as tumor suppressor genes, inhibited cell proliferation through different signaling pathways. Enforced expression of SOX7 and SOX17, both belonging to SOX subgroup F, played inhibitory roles in the growth and colony formation of EC cell in vitro by suppressing the accumulation of ݭcatenin in the Wnt/ݭcatenin signaling pathway19,21. SOX7 also inhibited the downstream factors of ݭcatenin, such as cyclinD1, c-Myc and fibroblast growth factor 9 (FGF9), in the Wnt/ݭcatenin signaling pathway19. Cell lines with elevated SOX17 expression also demonstrated high apoptosis and low proliferation rates through up-regulating wild-type p53, Bcl2-associated X protein, and cleaved caspase-3 and caspase-9, while simultaneously down-regulating the level of survivin and mastermind like-321,61. Conversely, down-regulation of SOX17 increased the rate of cell proliferation in cell lines, and suggested that the low expression level of SOX17 may be due to frequent mutations, including missense, frameshift, and hotspot missense changes. They also detected a moderate increase in ݭcatenin, as a key regulator of EC, following transfection of EC cell lines with mutated SOX1721,60. These results suggest that the regulation of SOX17 may vary in the progression of EC. In addition to the above genes, SOX15 is a novel and vital gene in EC. And the expression level of it was at significantly lower level in EC tissues compared with adjacent uninvolved tissues from the same patient75. And up-regulation of SOX15 in EC cell lines suppressed cell proliferation and viability by inducing cell-cycle arrest in G0/G1 stage, promoting cell apoptosis, and weakening cell migration, whereas knockout of SOX15 had the opposite effects. More researches are therefore needed to clarify the detailed mechanisms of SOX1575.
Apart from these oncogenes above, SOX2 and SOX9 are considered as bi-directionally regulated genes in EC. Overexpression of SOX2 in cells promoted cell proliferation by inhibiting expression of p2111, while low level of SOX2 in tissues caused by promoter hyper-methylation was conversely accompanied by initiation of EC58. These phenomena reflecting the role of SOX2 as a biomarker of clinical features in patients with EC was possibly due to different locations of SOX2 and its currently unclear mechanisms. Regarding SOX9, Behringer et al.71 found that overexpression of SOX9 in uterine epithelial cells in a progesterone receptor-Cre mouse model promoted the formation of more simple and complex cystic glandular structures. However, the results differed at the cellular level. Stable overexpression of SOX9 in EC cell lines served as a negative regulator of cell proliferation, particularly in the exponential growth phase, through activation of the p14ARF/p53/p21WAF1 pathway and interactions with nuclear factor-κB and Akt59. These results revealed that SOX9 behaved differently in vitro and in vivo, waiting for further investigations. The roles of SOX2 and SOX9 in EC thus remain largely uncertain.
Effects of SOX genes on treatment efficacy in EC
In relation to treatment of EC, only SOX17 was demonstrated an association with the chemosensitivity of EC cells to cisplatin. Zhang et al.61 overexpressed SOX17 in HEC-1B cells and found that cells with elevated expression of SOX17 had higher sensitivity to cisplatin, lower cell viability, and higher cell apoptosis rate when treated with cisplatin. These results suggest that SOX17 may be a promising target for gene treatment of EC.
Similarities of SOX genes in GC and prospective challenges
In this review, we classified SOX genes and emphasized their critical roles as regulators in the progression of GCs. And SOX genes were also considered as biomarkers of clinical features, including FIGO stage, histological grade, treatment efficacy, and prognosis of patients with GCs. SOX genes owned the potential to assist gynecologists in making precise clinical decisions. There were some similarities in SOX genes among GCs. 1) Methylation analysis of the tumor suppressor SOX1 gene in patients samples was regarded as a novel method for early screening and as a biomarker of clinical features. 2) Expression level of the dual-acting SOX2 gene revealed low level in some cases and high level in other cases, indicating different prognostic values in different patients, and highlighting the need for more research to clarify its role. 3) At a cellular level, transduction of the oncogene SOX4 in cell lines promoted the progression of GCs by enhancing cell proliferation and metastasis. 4) SOX7 and SOX17, both belong to subgroup F SOX genes, acted as tumor suppressor genes which were associated with decreased rate of progression. Given that SOX3 belongs to subgroup B with SOX1 and SOX2, meanwhile, SOX18 belongs to subgroup F with SOX7 and SOX17. Researchers could investigate the roles of SOX3 and SOX18 in GCs to see if they play similar roles to other members from the same subgroups.
The current review was limited to studies of the three most common GCs and certain histological subtypes, such as cervical squamous cell carcinoma, epithelial OC, and unexplained EC. Therefore, larger population-based studies of other kinds and subtypes of GCs, such as uterine myoma, uterine sarcoma, tumors of the fallopian tube, and vulvar squamous cell carcinoma, are warranted to further our understanding of the associations between SOX genes and the progression of GCs. Although studies to date only scratched the surface in terms of understanding the biological and clinical functions of SOX genes in GCs, these pre-clinical studies held promise and provided the basis for future studies aiming at elucidating the detailed functions of these genes.
Acknowledgments
This work was supported by grants from the National Natural Science Foundation of China (Grant No. 81572568 and 81272863).
Footnotes
↵*These authors contributed equally to this work.
Conflict of interest statement No potential conflicts of interest are disclosed.
- Received February 3, 2019.
- Accepted April 28, 2019.
- Copyright: © 2019, Cancer Biology & Medicine
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