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The BLM dissolvasome in DNA replication and repair

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Abstract

RecQ DNA helicases are critical for proper maintenance of genomic stability, and mutations in multiple human RecQ genes are linked with genetic disorders characterized by a predisposition to cancer. RecQ proteins are conserved from prokaryotes to humans and in all cases form higher-order complexes with other proteins to efficiently execute their cellular functions. The focus of this review is a conserved complex that is formed between RecQ helicases and type-I topoisomerases. In humans, this complex is referred to as the BLM dissolvasome or BTR complex, and is comprised of the RecQ helicase BLM, topoisomerase IIIα, and the RMI proteins. The BLM dissolvasome functions to resolve linked DNA intermediates without exchange of genetic material, which is critical in somatic cells. We will review the history of this complex and highlight its roles in DNA replication, recombination, and repair. Additionally, we will review recently established interactions between BLM dissolvasome and a second set of genome maintenance factors (the Fanconi anemia proteins) that appear to allow coordinated genome maintenance efforts between the two systems.

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Acknowledgments

We apologize to all authors whose work we could not cite due to space limitations. Work in our laboratory was funded by a grant from the National Institutes of Health (GM068061) and K.A.M. was supported in part by a National Institutes of Health training grant in Molecular Biosciences (GM07215).

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  1. Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI, 53706, USA

    Kelly A. Manthei & James L. Keck

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  1. Kelly A. Manthei
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Correspondence to James L. Keck.

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Manthei, K.A., Keck, J.L. The BLM dissolvasome in DNA replication and repair. Cell. Mol. Life Sci. 70, 4067–4084 (2013). https://doi.org/10.1007/s00018-013-1325-1

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  • DOI: https://doi.org/10.1007/s00018-013-1325-1

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