Grape Skin Polyphenols (GSPs) Inhibit the Migration of Mouse Mammary Carcinoma 4T1 Cells in Vitro

Qiu-yu Chen, Zhi-qiang Liang, Tao Sun, Xiang-jun Sun


OBJECTIVE    1) To study the efficacy of GSPs on the migration of highly metastatic mammary carcinoma cells and 2) To investigate inhibition mechanisms.
METHODS     Cell migration was assessed using a 24-well transwell assay. Cells with diff  erent concentrations of GSPs were suspended (5 × 105cell/mL) in RPMI 1640 media in the upper chamber, and RPMI 1640 media with 10% FBS was supplemented in the lower chamber. Then, cells were allowed to migrate for 24 h.
RESULTS    GSPs inhibited the migration of 4T1 cells in a dose-dependent manner. The migration of 4T1 cells was obviously inhibited by GSPs, even at a very low concentration (5 μg/mL), and was totally inhibited when the concentration was 20 μg/mL. Also, 20 μg/mL of GSPs inhibited cell viability by only 11.4% and induced early apoptosis by only 5.6% compared with a percentage of 4.0% in control cells. GSPs suppressed the activation of PDK1, Akt and Erk1/2 in a dose- dependent manner.
CONCLUSION    GSPs significantly inhibit the migration of highly metastatic mammary carcinoma 4T1 cells in vitro. This inhibition is independent of decreased cell viability or apoptosis induction. The inhibition of migration by GSPs is involved in blocking the PI3k/Akt and MAPK pathways.



grape skin polyphenols (GSPs), mammary carcinoma cells, migration, inhibition mechanism

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