Clonal Expansion and Cytotoxicity of TCRVl3 Subfamily T Cells Induced by CML and K562 Cells

Yuping ZHANG, Yangqiu LI, Shaohua CHEN, Lijian YANG, Gengxin LUO, Xueli ZHANG

Abstract


OBJECTIVE  To investigate  the  anti-leukemia effect, the  distribution  and clonal expansion of TCR V[3 subfamily T cells in T cells from cord blood and adult peripheral blood induced by CML cells and K562 cells in vitro.
METHODS  Peripheral blood T cells from  one adult donor and 3 cases of cord blood were stimulated with CML cells and K562 cells and further amplified by a suspended T cell-bulk culture,in order to induce CML specific cytotoxic T lymphocytes.  The induced  T cells were further  analyzed for  the specific cytotoxicity in CML by LDH assay, the phenotype identification  by indirect immunofluorescence  technique and the distribution and clonal expansion of TCR V~ subfamily by using reverse  transcriptase-polymerase chain reaction (RT-PCR) and genescan analysis, respectively.
RESULTS  Oligoclonal and oligoclonal tendency T cells with higher specific cytotoxicity from cord blood and adult peripheral blood could be induced by stimulation with CML cells and K562 cells.
CONCLUSIONS  Specific cytotoxic T cells for an anti-CML effect could be induced  by CML cells and K562 cells .The induced  T cells which have the characteristic  of specific cytotoxicity against CML cells may come from the clonal expansion of TCR V~, subfamily T cells.

Keywords


CML, T cell receptor, Vl3 subfamily, clonality, genescan.

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