Expression of the B-Cell Lymphoma/Leukemia 11A Gene in Malignant Hematological Cell Lines through Quantitative Reverse Transcription Polymerase Chain Reaction

Yang-jun Gao, Dong-mei He, Shao-hua Chen, Xiao-juan Yan, Xiao-mao Hu, Yang-qiu Li

Abstract


OBJECTIVE  The B-cell lymphoma/leukemia 11A (BCL11A) gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).
METHODS  The relative expression level of BCL11A mRNA in malignant hematological cell lines was determined through qRT-PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydro-genase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA.
RESULTS  The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute myeloid leukemia (P<0.05). Different cell lines with malignant B-cells exhibited a wide range of BCL11A expressions ranging from 27.37 to 93.38.
CONCLUSION  The overexpression of BCL11A gene mRNA in malignant B-cells might play a role in B-cell lymphoma/leukemia.

 


Keywords


B-cell lymphoma/leukemia 11A (BCL11A), malignant B-cells, real-time quantitative reverse transcription-polymerase chain reaction.

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