P2X7 Receptor Mediated Growth-Inhibitory Effect in KG1a Cell Line

Xiujun ZHANG, Lijun MENG

Abstract


OBJECTIVE    This study was conducted to investigate ATP induced growth inhibition in human leukemic cells KG1a.
METHODS    ATP inhibited cell growth was analyzed by MTS assay. Externalization of phosphatidylserine could be detected by Annexin-V-FITC apoptosis staining after activation of the P2X7 re-ceptor. P2X7 mediated pore formation was detected in KG1a cells by Yo-Pro-1 uptake assay.
RESULTS     ATP inhibited cell growth in a dose-dependent man-ner. The cytotoxic effect could be blocked by P2X7 antagonists, oxidized ATP (oATP) and KN62. Externalization of phosphatidyl-serine could be detected in a time-dependent manner. P2X7 medi-ated pore formation could be detected in KG1a cells. These eff ects could not be observed in P2X7 null Ramos cells.
CONCLUSION     The results and our previously reports that mRNA, protein expression and calcium response of the P2X7 receptor in KG1a cells, suggested that extracellular ATP eff ectively induces growth inhibition through apoptosis in KG1a cells by activation of P2X7 receptor, and that may be mediated by extracel-lular Ca2+ influx and pore formation.

Keywords


P2X7 receptor, growth inhibition, apoptosis, extracellular ATP, leukemia cells.

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