Detection of Human Papillomaviral Infection on Kazakh Patients with Esophageal Squamous Cell Carcinoma in Xinjiang

Ling CHEN, Lan YANG, Zhenzhu SUN, Haiyang ZHANG, Tao REN, Xiuyun TIAN, Liujuan PANG, Bin CHANG, Hongan LI, Feng LI

Abstract


OBJECTIVE    To investigate the detection rate of human papilloma virus (HPV) DNA in the Kazakh esophageal carcinoma (EC) patients of Xinjiang.
METHODS    We detected the prevalence of a HPV gene in tumor tissues from 318 esophageal squamous cell carcinoma (ESCC). Tumor tissues were kept in formalin and embedded in paraffin. One hundred seventeen samples used crude cell suspension, while the other 201 used the method of DNA extraction with phenol-Tris/chloroform. We analyzed the relevance to EC of Kazakh’s in Xinjiang.
RESULTS    In the ESCC samples of Kazakh’s in Xinjiang, total detection rate for HPV DNA was 64.5% (205/318). The positive rate of HPV in group of crude cell suspensions was 82.9% (97/117) compared with the rate of 53.7% (108/201) in the group of DNA extraction. The results in the two groups showed significant  diffference (χ2= 5.711,  P  < 0.05).
CONCLUSION    HPV DNA infection may be one of the most important factors related to EC of Kazakh’s in Xinjiang.

Keywords


HPV, EC, PCR, Kazakh.

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