HIF-1α siRNA Leads to Apoptosis of Pancreatic Cancer Cells under Hypoxic Conditions

Chuangui CHEN, Jianqiu CHEN, Jinjin SUN

Abstract


OBJECTIVE  To explore the role of hypoxic inducible factor-1α  (HIF-1 α ) in the proliferation and apoptosis of pancreatic cancer cells under hypoxic conditions.
METHODS  A cassette encoding small interference RNA (siRNA) targeting HIF-1 α mediated by recombinant adeno-associated virus (rAAV) was constructed, giving rAAV-siHIF. rAAV-siHIF or rAAV-hrGFP was transfected into exponentially growing MiaPaCa2 cells under hypoxic conditions. Then, the expression of HIF-1α  mRNA and protein, the proliferation and apoptosis of MiaPaCa2 cells were examined, using real-time PCR, Western Blot, MTT and TUNEL, respectively.
RESULTS  Under hypoxic conditions, rAAV-siHIF inhibited the expression of HIF-1 α  mRNA and protein in MiaPaCa2 cells. At the same time, rAAV-siHIF decreased MiaPaCa2 cell proliferation and induced apoptosis. However, rAAV-hrGFP had no effect on the expression of HIF-1 α  as well as the proliferation and apoptosis of MiaPaCa2 cells under hypoxic conditions.
CONCLUSION   Under hypoxic conditions, HIF-1α  plays a key role in the proliferation of MiaPaCa2 cells, and inhibition of HIF-1α  expression can lead to MiaPaCa2 cell apoptosis.

Keywords


recombinant adeno-associated virus (rAAV), hypoxia inducible factor (HIF),small interference RNA (siRNA), proliferation, apoptosis.

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