KIF18B promotes tumor progression in osteosarcoma by activating β-catenin

Tian Gao, Ling Yu, Zhiwei Fang, et al.

Abstract


Objective: Osteosarcoma is a common primary highly malignant bone tumor. Kinesin family member 18B (KIF18B) has beenidentified as a potential oncogene involved in the development and metastasis of several cancer types. While KIF18B overexpressionin osteosarcoma tissue is clearly detected, its specific function in the disease process remains to be established.

Methods: KIF18B expression was assessed in osteosarcoma tissues and cells. We additionally evaluated the effects of KIF18B onproliferation, migration, and invasion of osteosarcoma cells, both in vitro and in vivo.

Results: Our results showed overexpression of KIF18B in osteosarcoma tissues and cells. Knockdown of KIF18B induced G1/Sphase arrest and significantly inhibited proliferation, migration, and invasion of osteosarcoma cells, both in vitro and in vivo.KIF18B regulated β-catenin expression at the transcriptional level by controlling nuclear aggregation of ATF2 and at the posttranscriptionallevel by interacting with the adenomatous polyposis coli (APC) tumor suppressor gene in osteosarcoma cells.

Conclusions: KIF18B plays a carcinogenic role in osteosarcoma by regulating expression of β-catenin transcriptionally viadecreasing nuclear aggregation of ATF2 or post-transcriptionally through interactions with APC. Our collective findings support thepotential utility of KIF18B as a novel prognostic biomarker for osteosarcoma.

Cite this article as: Gao T, Yu L, Fang Z, Liu J, Bai C, Li S, et al. KIF18B promotestumor progression in osteosarcoma by activating β-catenin. Cancer Biol Med2020; 17: 371-386. doi: 10.20892/j.issn.2095-3941.2019.0452


Keywords


β-catenin; APC; ATF2; KIF18B; osteosarcoma

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