LncRNA PVT1 promotes ovarian cancer progression by silencing miR-214

Ying Chen, et al.

Abstract


Objective: Emerging evidence indicates that long non-coding RNAs (lncRNAs) are critical in carcinogenesis and progression ofovarian cancer. This study aimed to explore the functions and molecular mechanisms of plasmacytoma variant translocation I(PVT1) in ovarian cancer

Methods: PVT1 and miR-214 were detected by qRT-PCR assays in ovarian cancer tissues and cells. The cell proliferation,migration, and invasion abilities were detected by cell functional experiments, respectively. Western blot assay was performed todetect epithelial-mesenchymal transition (EMT) markers. MiR-214 expression regulated by PVT1 was studied by RNAimmunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays.

Results: The expression of PVT1 was up-regulated in ovarian cancer tissues and cell lines. Elevated PVT1 expression wasassociated with advanced stage and indicated poor prognosis for ovarian cancer patients. The knockdown of PVT1 impairedSKOV3 cell proliferation, migration, and invasion in vitro. The promotion of ovarian cancer progression by PVT1 involved inregulation of the epithelial-mesenchymal transition process and PVT1 interaction with EZH2 represses miR-214 expression inovarian cancer cells.

Conclusions: PVT1 plays an important role in ovarian cancer tumorigenesis, which might be as a novel diagnostic marker andtherapeutic target for ovarian cancer.


Keywords


Long non-coding RNA; PVT1; ovarian cancer; miR-214

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