PT  - JOURNAL ARTICLE
AU  - GAO, Yang-jun
AU  - HE, Dong-mei
AU  - CHEN, Shao-hua
AU  - YAN, Xiao-juan
AU  - HU, Xiao-mao
AU  - LI, Yang-qiu
TI  - Expression of the B-Cell Lymphoma/Leukemia 11A Gene in Malignant Hematological Cell Lines through Quantitative Reverse Transcription Polymerase Chain Reaction
AID  - 10.1007/s11805-011-0588-2
DP  - 2011 Dec 01
TA  - Clinical Oncology and Cancer Research
PG  - 242--246
VI  - 8
IP  - 4
4099  - http://www.cancerbiomed.org/content/8/4/242.short
4100  - http://www.cancerbiomed.org/content/8/4/242.full
SO  - Cancer Biol Med2011 Dec 01; 8
AB  - OBJECTIVE The B-cell lymphoma/leukemia 11A (BCL11A) gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).METHODS The relative expression level of BCL11A mRNA in malignant hematological cell lines was determined through qRT-PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydrogenase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA.RESULTS The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute myeloid leukemia (P < 0.05). Different cell lines with malignant B-cells exhibited a wide range of BCL11A expressions ranging from 27.37 to 93.38.CONCLUSION The overexpression of BCL11A gene mRNA in malignant B-cells might play a role in B-cell lymphoma/leukemia.