PT  - JOURNAL ARTICLE
AU  - Zhang, Xiujun
AU  - Meng, Lijun
TI  - P2X7 Receptor Mediated Growth-Inhibitory Effect in KG1a Cell Line
AID  - 10.1007/s11805-008-0400-0
DP  - 2008 Dec 01
TA  - Chinese Journal of Clinical Oncology
PG  - 400--406
VI  - 5
IP  - 6
4099  - http://www.cancerbiomed.org/content/5/6/400.short
4100  - http://www.cancerbiomed.org/content/5/6/400.full
SO  - Cancer Biol Med2008 Dec 01; 5
AB  - OBJECTIVE This study was conducted to investigate ATP-induced growth inhibition in human leukemic cells KG1a.METHODS ATP inhibited cell growth was analyzed by MTS assay. Externalization of phosphatidylserine could be detected by Annexin-V-FITC apoptosis staining after activation of the P2X7 receptor. P2X7 mediated pore formation was detected in KG1a cells by Yo-Pro-1 uptake assay.RESULTS ATP inhibited cell growth in a dose-dependent manner. The cytotoxic effect could be blocked by P2X7 antagonists, oxidized ATP (oATP) and KN62. Externalization of phosphatidylserine could be detected in a time-dependent manner. P2X7 mediated pore formation could be detected in KG1a cells. These effects could not be observed in P2X7 null Ramos cells.CONCLUSION The results and our previously reports that mRNA, protein expression and calcium response of the P2X7 receptor in KG1a cells, suggested that extracellular ATP effectively induces growth inhibition through apoptosis in KG1a cells by activation of P2X7 receptor, and that may be mediated by extracellular Ca2+ influx and pore formation.